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Canine Cluster Of Differentiation 40 Ligand (CD40L) ELISA Kit

SKU:
CNDL00018
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O97626
Sensitivity:
0.032ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CD154,TRAP,HIGM1,IGM,IMD3,TBAM,T-BAM,TNFSF5,Gp39,TNF Superfamily Member 5,Hyper-IgM Syndrome,TNF-Related Activation Protein,T-Cell B-Cell Activating Molecule
Reactivity:
Canine
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

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Canine Cluster Of Differentiation 40 Ligand (CD40L) ELISA Kit

The Canine Cluster of Differentiation 40 Ligand (CD40L) ELISA Kit is specifically designed for the precise measurement of CD40L levels in canine serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit ensures accurate and reproducible results, making it ideal for various research purposes.CD40L, also known as CD154, is a critical protein involved in immune responses and inflammation. It plays a crucial role in regulating the immune system and is linked to various diseases, including autoimmune disorders and chronic inflammatory conditions.

As a valuable biomarker, studying CD40L levels can provide insights into these diseases and potentially guide the development of targeted therapies. Overall, the Canine CD40L ELISA Kit offers researchers a reliable tool for investigating the role of CD40L in canine health and disease, facilitating advancements in veterinary medicine and immunology research.

Product Name:Canine Cluster Of Differentiation 40 Ligand (CD40L) ELISA Kit
Product Code:CNDL00018
Size:96 Assays
Target:CD40L
Synonyms:CD154, TRAP, HIGM1, IGM, IMD3, TBAM, T-BAM, TNFSF5, Gp39, TNF Superfamily Member 5, Hyper-IgM Syndrome, TNF-Related Activation Protein, T-Cell B-Cell Activating Molecule
Detection Method:Sandwich
Range:0.156-10 ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)91-9894
Heparin plasma (n=5)90-9994
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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