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Canine Brain Natriuretic Peptide (BNP) ELISA Kit

SKU:
CNDL00012
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
F6XBK9
Sensitivity:
13.4pg/mL
Range:
31.25-2000pg/mL
ELISA Type:
Sandwich
Synonyms:
GC-B,B-Type Natriuretic Peptide,Ventricular Natriuretic Peptide,Gamma-brain natriuretic peptide,Brain natriuretic peptide 32
Reactivity:
Canine
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
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Description

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Canine Brain Natriuretic Peptide (BNP) ELISA Kit

The Canine Brain Natriuretic Peptide (BNP) ELISA Kit is specifically designed for the accurate detection of BNP levels in canine serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications. BNP is a peptide hormone released by the heart in response to increased cardiac stress or pressure. Elevated levels of BNP are associated with heart failure, making it a valuable biomarker for diagnosing and monitoring cardiac conditions in dogs.

This kit is essential for researchers and veterinarians studying cardiovascular diseases in canines, offering a powerful tool for understanding the pathophysiology of heart conditions and developing effective treatment strategies. With its superior performance, the Canine BNP ELISA Kit is a valuable asset for advancing canine health research.

Product Name:Canine Brain Natriuretic Peptide (BNP) ELISA Kit
Product Code:CNDL00012
Size:96 Assays
Target:BNP
Synonyms:GC-B, B-Type Natriuretic Peptide, Ventricular Natriuretic Peptide, Gamma-brain natriuretic peptide, Brain natriuretic peptide 32
Detection Method:Sandwich
Range:31.25-2000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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