The CAND1 Polyclonal Antibody (CAB14287) is a valuable tool for researchers studying the Cullin-associated Nedd8-dissociated protein 1 (CAND1) protein. CAND1 is involved in the regulation of Cullin-RING ubiquitin ligase (CRL) complexes, making it critical for the control of protein degradation and cell cycle progression. This polyclonal antibody, generated in rabbits, is highly specific for human samples and is validated for use in Western blotting applications.By binding to CAND1, this antibody enables the detection and analysis of the protein in various cell types, making it ideal for studies in the fields of cell biology and cancer research.
Understanding the role of CAND1 in protein degradation pathways and cell cycle regulation is essential for developing targeted therapies that manipulate these processes in diseases such as cancer and neurodegenerative disorders. Adding the CAND1 Polyclonal Antibody to your research arsenal will provide crucial insights into the mechanisms underlying these conditions.
Product Name:
CAND1 Rabbit Polyclonal Antibody
SKU:
CAB14287
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 750-980 of human CAND1 (NP_060918.2).
This gene encodes an essential regulator of Cullin-RING ubiquitin ligases, which are in involved in ubiquitinylation of proteins degraded by the Ub proteasome system. The encoded protein binds to unneddylated cullin-RING box protein complexes and acts as an inhibitor of cullin neddylation and of Skp1, cullin, and F box ubiquitin ligase complex assembly and activity. In mammalian cell culture, this protein predominantly localizes to the cytoplasm. Knockdown of this gene in preadipocytes results in blocked adipogenesis. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
55832
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using CAND1 Rabbit pAb (CAB14287) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 15s.
