The CALR3 Polyclonal Antibody (PAC043696) is a valuable tool for researchers studying CALR3, a member of the calreticulin family involved in various cellular functions. This antibody, produced in rabbits, exhibits high reactivity towards human samples and has been validated for use in Western blot applications. By binding to the CALR3 protein, this antibody facilitates the detection and analysis of CALR3 in different cell types, making it suitable for immunology and cancer research studies.CALR3, a protein closely related to calreticulin, is known to play a role in calcium homeostasis and protein quality control within the endoplasmic reticulum.
Its involvement in cellular processes such as protein folding, calcium buffering, and signal transduction highlights its importance in maintaining cellular function and response to stress. Research focusing on CALR3 may provide insights into its role in diseases like cancer, neurodegenerative disorders, and cardiovascular conditions, offering potential therapeutic targets for intervention.By utilizing the CALR3 Polyclonal Antibody in experimental studies, researchers can further explore the functions and mechanisms of CALR3 in various biological contexts, advancing our understanding of its implications in health and disease.
Antibody Name:
CALR3 Antibody (PACO43696)
Antibody SKU:
PACO43696
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:10000, IHC:1:20-1:200
Species Reactivity:
Human
Immunogen:
Recombinant Human Calreticulin-3 protein (135-384AA)
Form:
Liquid
Storage Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification Method:
Antigen Affinity Purified
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Immunohistochemistry of paraffin-embedded human breast cancer using PACO43696 at dilution of 1:100.
Immunohistochemistry of paraffin-embedded human testis tissue using PACO43696 at dilution of 1:100.
Background:
During spermatogenesis, may act as a lectin-independent chaperone for specific client proteins such as ADAM3. Required for sperm fertility (By similarity). CALR3 capacity for calcium-binding may be absent or much lower than that of CALR.
calreticulin 3: During spermatogenesis, may act as a lectin-independent chaperone for specific client proteins such as ADAM3. Required for sperm fertility. CALR3 capacity for calcium- binding may be absent or much lower than that of CALR. Defects in CALR3 are the cause of familial hypertrophic cardiomyopathy type 19 (CMH19). CMH19 is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. Belongs to the calreticulin family.Protein type: Chaperone; Cancer Testis Antigen (CTA)Chromosomal Location of Human Ortholog: 19p13.11Cellular Component: endoplasmic reticulum lumenMolecular Function: unfolded protein binding; calcium ion binding; carbohydrate bindingBiological Process: protein folding; spermatogenesis; cell differentiationDisease: Cardiomyopathy, Familial Hypertrophic, 19
UniProt Protein Details:
NCBI Summary:
The protein encoded by this gene belongs to the calreticulin family, members of which are calcium-binding chaperones localized mainly in the endoplasmic reticulum. This protein is also localized to the endoplasmic reticulum lumen, however, its capacity for calcium-binding may be absent or much lower than other family members. This gene is specifically expressed in the testis, and may be required for sperm fertility. Mutation in this gene has been associated with familial hypertrophic cardiomyopathy. [provided by RefSeq, Dec 2011]
