The Calreticulin 3 (CALR3) Polyclonal Antibody (PAC043695) is a valuable tool for researchers studying CALR3, a calcium-binding chaperone protein involved in cellular processes such as protein folding and calcium homeostasis. This rabbit polyclonal antibody is highly specific to human samples and has been validated for use in Western blot applications. By binding to CALR3, this antibody enables the detection and analysis of CALR3 protein in various cell types, making it ideal for research in cell biology and cancer studies.
CALR3, a member of the calreticulin family, is known to play a role in cellular functions such as calcium storage and signaling, as well as protein quality control. Dysregulation of CALR3 has been linked to diseases including cancer and neurodegenerative disorders, making it an important target for further investigation in the field of molecular biology and biomedical research. Understanding the function and regulation of CALR3 can provide valuable insights into disease mechanisms and potential therapeutic strategies.
Recombinant Human Calreticulin-3 protein (135-384AA)
Form:
Liquid
Storage Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification Method:
Antigen Affinity Purified
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot. All lanes: Calreticulin-3 antibody at 4µg/ml. Lane 1: Hela whole cell lysate. Lane 2: HepG2 whole cell lysate. Lane 3: Rat gonad tissue. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 45 kDa. Observed band size: 45 kDa.
Immunohistochemistry of paraffin-embedded human testis tissue using PACO43695 at dilution of 1:100.
Immunohistochemistry of paraffin-embedded human breast cancer using PACO43695 at dilution of 1:100.
Background:
During spermatogenesis, may act as a lectin-independent chaperone for specific client proteins such as ADAM3. Required for sperm fertility (By similarity). CALR3 capacity for calcium-binding may be absent or much lower than that of CALR.
calreticulin 3: During spermatogenesis, may act as a lectin-independent chaperone for specific client proteins such as ADAM3. Required for sperm fertility. CALR3 capacity for calcium- binding may be absent or much lower than that of CALR. Defects in CALR3 are the cause of familial hypertrophic cardiomyopathy type 19 (CMH19). CMH19 is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. Belongs to the calreticulin family.Protein type: Chaperone; Cancer Testis Antigen (CTA)Chromosomal Location of Human Ortholog: 19p13.11Cellular Component: endoplasmic reticulum lumenMolecular Function: unfolded protein binding; calcium ion binding; carbohydrate bindingBiological Process: protein folding; spermatogenesis; cell differentiationDisease: Cardiomyopathy, Familial Hypertrophic, 19
UniProt Protein Details:
NCBI Summary:
The protein encoded by this gene belongs to the calreticulin family, members of which are calcium-binding chaperones localized mainly in the endoplasmic reticulum. This protein is also localized to the endoplasmic reticulum lumen, however, its capacity for calcium-binding may be absent or much lower than other family members. This gene is specifically expressed in the testis, and may be required for sperm fertility. Mutation in this gene has been associated with familial hypertrophic cardiomyopathy. [provided by RefSeq, Dec 2011]
