The Calpain 3 Polyclonal Antibody (CAB11995) is a valuable tool for researchers studying calpain 3, a calcium-dependent protease involved in muscle cell function and maintenance. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the calpain 3 protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it ideal for investigations in muscle biology and related areas of research.Calpain 3, also known as p94, is essential for the maintenance of muscle integrity and function, playing a key role in processes such as muscle repair and regeneration.
Dysregulation of calpain 3 has been linked to various muscle disorders, making it a promising target for studying muscular dystrophies and other muscle-related diseases. Understanding the mechanisms behind calpain 3 activity is crucial for developing potential therapeutic interventions that could benefit individuals affected by these conditions. Choose the Calpain 3 Polyclonal Antibody for reliable and accurate detection of calpain 3 in your research studies.
Product Name:
CAPN3 Rabbit Polyclonal Antibody
SKU:
CAB11995
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human CAPN3 (NP_775111.1).
Calpain, a heterodimer consisting of a large and a small subunit, is a major intracellular protease, although its function has not been well established. This gene encodes a muscle-specific member of the calpain large subunit family that specifically binds to titin. Mutations in this gene are associated with limb-girdle muscular dystrophies type 2A. Alternate promoters and alternative splicing result in multiple transcript variants encoding different isoforms and some variants are ubiquitously expressed.
Purification Method:
Affinity purification
Gene ID:
825
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using CAPN3 Rabbit pAb (CAB11995) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
