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Calpain 2 Polyclonal Antibody (CAB2671)

SKU:
CAB2671
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
IHC
€129 - €359
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Calpain 2 Polyclonal Antibody
SKU:CAB2671
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 520-700 of human Calpain 2 (NP_001739.2).
Sequence:LEEF DISE DDID DGFR RLFA QLAG EDAE ISAF ELQT ILRR VLAK RQDI KSDG FSIE TCKI MVDM LDSD GSGK LGLK EFYI LWTK IQKY QKIY REID VDRS GTMN SYEM RKAL EEAG FKMP CQLH QVIV ARFA DDQL IIDF DNFV RCLV RLET LFKI FKQL DPEN TGTI ELDL ISWL CFSV L
Tested Applications:WB IHC-P ELISA
Recommended Dilution:WB,1:500 - 1:1000 IHC-P,1:50 - 1:200
Synonyms:CANP2; mCANP; CANPL2; CANPml; Calpain 2
Positive Sample:293T,Mouse brain,Mouse skin
Conjugate:Unconjugated
Cellular Localization:Cell membrane, Cytoplasm.
Calculated MW:80kDa
Observed MW:80kDa

The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene.

Purification Method:Affinity purification
Gene ID:824
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using Calpain 2 Rabbit pAb (CAB2671).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.

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