The Calpain 10 Polyclonal Antibody (CAB9865) is a high-quality antibody designed for research involving Calpain 10, a protein that plays a key role in regulating cellular processes such as calcium signaling and insulin secretion. This antibody, raised in rabbits, is highly specific and reactive with human samples, making it ideal for use in Western blot applications.Calpain 10 is a member of the calpain family of proteases and is known to be involved in various cellular functions, including cell cycle progression and apoptosis. Dysregulation of Calpain 10 has been implicated in diseases such as diabetes and neurodegenerative disorders, making it an important target for research in these areas.
By using the Calpain 10 Polyclonal Antibody, researchers can detect and analyze the expression of Calpain 10 in different cell types, allowing for a better understanding of its role in various biological processes. This antibody is a valuable tool for studies in the fields of cell biology, molecular biology, and disease research, providing insights that may lead to the development of new therapeutic strategies.
Product Name:
CAPN10 Rabbit Polyclonal Antibody
SKU:
CAB9865
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human CAPN10 (NP_075571.1).
Calpains represent a ubiquitous, well-conserved family of calcium-dependent cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large catalytic subunit has four domains: domain I, the N-terminal regulatory domain that is processed upon calpain activation; domain II, the protease domain; domain III, a linker domain of unknown function; and domain IV, the calmodulin-like calcium-binding domain. This gene encodes a large subunit. It is an atypical calpain in that it lacks the calmodulin-like calcium-binding domain and instead has a divergent C-terminal domain. It is similar in organization to calpains 5 and 6. This gene is associated with type 2 or non-insulin-dependent diabetes mellitus (NIDDM), and is located within the NIDDM1 region. Multiple alternative transcript variants have been described for this gene.
Purification Method:
Affinity purification
Gene ID:
11132
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using CAPN10 antibody (CAB9865) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
