The Calpain-1 Rabbit Monoclonal Antibody (CAB8710) is a highly specific and sensitive tool for studying the protein calpain-1 in various research applications. Calpain-1 is a calcium-dependent protease that plays a crucial role in numerous cellular processes, including cell signaling, cytoskeletal remodeling, and apoptosis.This antibody, produced using rabbit monoclonal technology, has been validated for use in Western blotting, immunohistochemistry, and other laboratory techniques. It specifically binds to calpain-1, allowing for accurate detection and quantification in samples from different cell types and tissues.
Researchers in the fields of cell biology, neuroscience, and cancer research can benefit from using this antibody to investigate the functions of calpain-1 in normal physiological processes and disease states. By understanding the role of calpain-1, scientists can uncover potential therapeutic targets for conditions such as neurodegenerative diseases, cardiovascular disorders, and cancer.
Product Name:
Calpain 1 Rabbit Monoclonal Antibody
SKU:
CAB8710
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human Calpain 1 (P07384).
The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
823
Clone Number:
ARC1272
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Calpain 1 Rabbit mAb (CAB8710) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.
