Calpain 1 Polyclonal Antibody (CAB21673)
- SKU:
- CAB21673
- Product Type:
- Antibody
- Antibody Type:
- Polyclonal Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- WB
Frequently bought together:
Description
| Product Name: | Calpain 1 Polyclonal Antibody |
| SKU: | CAB21673 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-330 of human Calpain 1 (NP_005177.2). |
| Sequence: | KQRA RELG LGRH ENAI KYLG QDYE QLRV RCLQ SGTL FRDE AFPP VPQS LGYK DLGP NSSK TYGI KWKR PTEL LSNP QFIV DGAT RTDI CQGA LGDC WLLA AIAS LTLN DTLL HRVV PHGQ SFQN GYAG IFHF QLWQ FGEW VDVV VDDL LPIK DGKL VFVH SAEG NEFW SALL EKAY AKVN GSYE ALSG GSTS EGFE DFTG GVTE WYEL RKAP SDLY QIIL KALE RGSL LGCS IDIS SVLD MEAI TFKK LVKG HAYS VTGA KQVN YRGQ VVSL IRMR NPWG EVEW TGAW SDSS SEWN NVDP YERD QLRV KME |
| Tested Applications: | WB ELISA |
| Recommended Dilution: | WB,1:500 - 1:2000 |
| Synonyms: | CANP; muCL; CANP1; SPG76; CANPL1; muCANP; Calpain 1 |
| Positive Sample: | SW480,A549,BT-474,MCF7,Jurkat |
| Conjugate: | Unconjugated |
| Cellular Localization: | Cell membrane, Cytoplasm. |
| Calculated MW: | 82kDa |
| Observed MW: | Refer to figures |
The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene.
| Purification Method: | Affinity purification |
| Gene ID: | 823 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
 | Western blot analysis of various lysates using Calpain 1 Rabbit pAb (CAB21673) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST. |
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