The Caldesmon Polyclonal Antibody (CAB5366) is a valuable tool for researchers studying caldesmon, a protein involved in the regulation of smooth muscle contraction and cell movement. This antibody, produced in rabbits, is highly specific to human caldesmon and has been validated for use in Western blot applications.Caldesmon is known to play a crucial role in the control of actin-myosin interactions, making it essential for processes such as muscle contraction and cell migration. Dysregulation of caldesmon has been linked to various diseases, including cancer and cardiovascular disorders, highlighting its importance in biomedical research.
By using the Caldesmon Polyclonal Antibody (CAB5366), researchers can accurately detect and analyze caldesmon expression in different cell types, providing valuable insights into its function and potential therapeutic applications. This antibody is a valuable tool for studies investigating smooth muscle function, cell motility, and disease mechanisms related to caldesmon dysregulation.
Product Name:
CALD1 Rabbit Polyclonal Antibody
SKU:
CAB5366
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human CALD1 (NP_004333.1).
This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.
Purification Method:
Affinity purification
Gene ID:
800
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from A-431 cells, using CALD1 Rabbit pAb (CAB5366) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
