The Cadherin-1 Polyclonal Antibody (CAB3044) is a valuable tool for research involving Cadherin-1, a cell adhesion molecule that plays a crucial role in cell-cell adhesion and signaling. This antibody, raised in rabbits, is highly specific and sensitive for detecting Cadherin-1 in human samples, making it ideal for use in Western blot applications. Cadherin-1, also known as E-cadherin, is essential for maintaining tissue integrity and organization, and its dysregulation is associated with carcinogenesis and metastasis in various cancers. By targeting Cadherin-1, researchers can gain insights into the molecular mechanisms underlying tumor progression and potentially discover new therapeutic targets for cancer treatment.
With its high reactivity and specificity, the Cadherin-1 Polyclonal Antibody is a valuable tool for studying the role of Cadherin-1 in cancer biology, development, and tissue homeostasis. It is a crucial reagent for researchers in the fields of cell biology, oncology, and molecular biology who are investigating Cadherin-1 function and its implications in disease.
Product Name:
E-Cadherin Polyclonal Antibody
SKU:
CAB3044
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 373-622 of human E-Cadherin (NP_004351.1).
Cell junction, Cell membrane, Endosome, Golgi apparatus, Single-pass type I membrane protein, trans-Golgi network.
Calculated MW:
97kDa
Observed MW:
125kDa
This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16.
Purification Method:
Affinity purification
Gene ID:
999
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using E-Cadherin Rabbit pAb (CAB3044) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
