The Cadherin-1 Polyclonal Antibody (CAB11492) is a vital tool for research involving Cadherin-1, a key cell adhesion molecule crucial for cell-cell interactions. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to Cadherin-1 protein, this antibody enables precise detection and analysis in various cell types, making it an excellent choice for studies in cell biology, tissue development, and cancer research.Cadherin-1, also known as E-cadherin, plays a pivotal role in maintaining tissue integrity and regulating cell proliferation and migration.
Dysregulation of Cadherin-1 expression has been implicated in various diseases, including cancer metastasis and developmental disorders. Understanding the functions and mechanisms of Cadherin-1 is crucial for unraveling its contributions to disease pathogenesis and identifying potential therapeutic targets.Overall, the Cadherin-1 Polyclonal Antibody (CAB11492) serves as a valuable tool for researchers seeking to delve deeper into the roles of Cadherin-1 in health and disease, paving the way for new insights and potential treatment strategies.
Product Name:
E-Cadherin Rabbit Polyclonal Antibody
SKU:
CAB11492
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 373-622 of human E-Cadherin (NP_004351.1).
Cell junction, Cell membrane, Endosome, Golgi apparatus, Single-pass type I membrane protein, trans-Golgi network.
Calculated MW:
97kDa
Observed MW:
125kDa/135kDa
This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16.
Purification Method:
Affinity purification
Gene ID:
999
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HCT116 cells, using E-Cadherin Rabbit pAb (CAB11492) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
