The CAD Polyclonal Antibody (CAB8344) is a crucial tool for researchers studying CAD, a key enzyme in the pyrimidine biosynthesis pathway. The antibody, produced in rabbits, shows high reactivity with CAD protein in human samples and is validated for use in Western blotting applications. By targeting the CAD protein, this antibody allows for precise detection and analysis in various cell types, making it an invaluable asset for research in molecular biology and drug development.CAD, or carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase, is essential for the de novo synthesis of pyrimidines, which are building blocks for DNA and RNA.
Dysregulation of CAD activity has been linked to a variety of diseases, including cancer, genetic disorders, and neurodegenerative conditions. Understanding the function and regulation of CAD is crucial for developing targeted therapies that can manipulate pyrimidine metabolism in disease settings.The CAD Polyclonal Antibody (CAB8344) provides researchers with a powerful tool to investigate the role of CAD in cellular processes and disease pathways. Its specificity and sensitivity make it an ideal choice for studies exploring the mechanisms of pyrimidine biosynthesis, as well as potential therapeutic interventions targeting CAD activity.
Product Name:
CAD Rabbit Polyclonal Antibody
SKU:
CAB8344
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1650-1900 of human CAD (NP_004332.2).
The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
790
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using CAD Rabbit pAb (CAB8344) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
