The CABIN1 Polyclonal Antibody (PACO60901) is a crucial tool for researchers studying the CABIN1 protein, a key regulator of transcription and chromatin remodeling. This antibody, produced in rabbits, is highly specific to human samples and is validated for use in Western blotting applications. By binding to the CABIN1 protein, it enables precise detection and analysis in a variety of cell types, making it an essential component for studies in molecular biology and cancer research.CABIN1 is known to interact with multiple proteins involved in gene expression regulation, making it a central player in controlling cellular processes such as proliferation and differentiation.
Understanding the function and regulation of CABIN1 is essential for unraveling its role in signaling pathways and its potential implications in diseases like cancer and neurological disorders. This antibody provides researchers with a reliable tool to explore the intricate mechanisms involving CABIN1, ultimately advancing our knowledge of cellular function and disease pathology.
IHC image of PACO60901 diluted at 1:400 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot. Positive WB detected in: A549 whole cell lysate, Rat brain tissue. All lanes: CABIN1 antibody at 3.1µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 247, 238 kDa. Observed band size: 247 kDa.
IHC image of PACO60901 diluted at 1:400 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
May be required for replication-independent chromatin assembly. May serve as a negative regulator of T-cell receptor (TCR) signaling via inhibition of calcineurin. Inhibition of activated calcineurin is dependent on both PKC and calcium signals. Acts as a negative regulator of p53/TP53 by keeping p53 in an inactive state on chromatin at promoters of a subset of it's target genes.
Synonyms:
Calcineurin-binding protein cabin-1 (Calcineurin inhibitor) (CAIN), CABIN1, KIAA0330
UniProt Protein Function:
May be required for replication-independent chromatin assembly. May serve as a negative regulator of T-cell receptor (TCR) signaling via inhibition of calcineurin. Inhibition of activated calcineurin is dependent on both PKC and calcium signals. Acts as a negative regulator of p53/TP53 by keeping p53 in an inactive state on chromatin at promoters of a subset of it's target genes.
NCBI Summary:
Calcineurin plays an important role in the T-cell receptor-mediated signal transduction pathway. The protein encoded by this gene binds specifically to the activated form of calcineurin and inhibits calcineurin-mediated signal transduction. The encoded protein is found in the nucleus and contains a leucine zipper domain as well as several PEST motifs, sequences which confer targeted degradation to those proteins which contain them. Alternative splicing results in multiple transcript variants encoding two different isoforms. [provided by RefSeq, Jan 2011]
