The CA9 Rabbit Monoclonal Antibody (CAB4934) is a cutting-edge product specifically designed for research involving CA9, a cell surface protein involved in various cellular functions, including pH regulation and angiogenesis. This highly specific antibody, developed using rabbit monoclonal technology, is ideal for use in a variety of experimental applications, including immunohistochemistry and flow cytometry.CA9, also known as carbonic anhydrase IX, is a key player in tumor microenvironment regulation and has been implicated in promoting tumor growth and metastasis. The CA9 Rabbit Monoclonal Antibody (CAB4934) offers researchers a powerful tool for studying the expression and function of CA9 in cancer biology and other related fields.
Its high sensitivity and specificity ensure accurate and reliable results, making it an essential component for studies focused on understanding the role of CA9 in disease progression and therapeutic development.Overall, the CA9 Rabbit Monoclonal Antibody (CAB4934) is a valuable asset for researchers looking to investigate the intricate mechanisms underlying CA9's involvement in disease pathology and for those seeking to develop targeted therapies that exploit CA9 as a potential therapeutic target.
Cell membrane, Cell projection, Nucleus, Single-pass type I membrane protein, microvillus membrane, nucleolus.
Calculated MW:
50kDa
Observed MW:
35-58kDa
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12.
Purification Method:
Affinity purification
Gene ID:
768
Clone Number:
ARC1275
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using Carbonic Anhydrase 9 (CA9/G250) Rabbit mAb (CAB4934) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
