The CA5B Polyclonal Antibody (CAB17633) is specifically designed for research involving CA5B, a crucial enzyme involved in the regulation of pH balance in cells. This antibody, raised in rabbits, is highly reactive with human samples and has been validated for use in various applications, including Western blot and immunohistochemistry.CA5B plays a key role in catalyzing the reversible hydration of carbon dioxide to bicarbonate, which is essential for maintaining acid-base balance in cells. Its dysregulation has been implicated in various diseases, including metabolic disorders and cancer.
Understanding the function and expression of CA5B is important for advancing research in these areas and potentially developing targeted therapies.This polyclonal antibody binds specifically to the CA5B protein, allowing for accurate detection and analysis in different cell types and tissues. Its reliability and sensitivity make it an ideal tool for investigating the role of CA5B in physiological and pathological processes, making it a valuable asset for researchers in the fields of biochemistry, molecular biology, and medicine.
Product Name:
CA5B Rabbit Polyclonal Antibody
SKU:
CAB17633
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-317 of human CA5B (NP_009151.1).
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This gene encodes carbonic anhydrase 5B. CA5B, and the related CA5A gene, has its expression localized in the mitochondria though CA5B has a wider tissue distribution than CA5A, which is restricted to the liver, kidneys, and skeletal muscle. A carbonic anhydrase pseudogene (CA5BP1) is adjacent to the CA5B gene and these two loci produce CA5BP1-CA5B readthrough transcripts.
Purification Method:
Affinity purification
Gene ID:
11238
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates, using CA5B Rabbit pAb (CAB17633) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
