The CA3 Polyclonal Antibody (CAB1212) is a research tool designed for studying the CA3 protein, an important enzyme involved in various biological processes such as cell metabolism and signaling. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the CA3 protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it ideal for research in fields such as biochemistry and molecular biology.
CA3, also known as carbonic anhydrase III, plays a crucial role in maintaining pH homeostasis and is implicated in conditions such as metabolic disorders and certain cancers. Studying the function and regulation of CA3 can provide insight into its potential role in disease pathogenesis, paving the way for the development of targeted therapies and diagnostic tools. By utilizing the CA3 Polyclonal Antibody, researchers can further explore the intricate mechanisms underlying CA3 activity and its impact on human health.
Product Name:
CA3 Rabbit Polyclonal Antibody
SKU:
CAB1212
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human CA3 (NP_005172.1).
Carbonic anhydrase III (CAIII) is a member of a multigene family (at least six separate genes are known) that encodes carbonic anhydrase isozymes. These carbonic anhydrases are a class of metalloenzymes that catalyze the reversible hydration of carbon dioxide and are differentially expressed in a number of cell types. The expression of the CA3 gene is strictly tissue specific and present at high levels in skeletal muscle and much lower levels in cardiac and smooth muscle. A proportion of carriers of Duchenne muscle dystrophy have a higher CA3 level than normal. The gene spans 10.3 kb and contains seven exons and six introns.
Purification Method:
Affinity purification
Gene ID:
761
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from Rat skeletal muscle, using CA3 Rabbit pAb (CAB1212) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
