The C4C Polyclonal Antibody is a valuable tool for researchers studying the C4C protein, a key player in immune regulation and inflammation. This antibody, generated in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the C4C protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it an ideal choice for studies in immunology and cancer research.C4C, also known as an immune regulatory molecule, is involved in modulating immune responses and maintaining immune balance.
Research into the role of C4C is important in understanding how the immune system functions in health and disease, particularly in conditions such as cancer, autoimmune disorders, and chronic inflammation. By gaining insights into the function of C4C, researchers can work towards developing targeted therapies that manipulate the immune system for therapeutic benefit.
Product Name:
C4c Polyclonal Antibody
SKU:
CAB24351
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1337-1446 of human C4c (NP_001002029.3).
Hep G2,Mouse liver,Mouse spleen,Rat liver,Rat thymus
Conjugate:
Unconjugated
Calculated MW:
192kDa
Observed MW:
85kDa
This gene encodes the basic form of complement factor 4, and together with the C4A gene, is part of the classical activation pathway. The protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains prior to secretion. The trimer provides a surface for interaction between the antigen-antibody complex and other complement components. The alpha chain may be cleaved to release C4 anaphylatoxin, a mediator of local inflammation. Deficiency of this protein is associated with systemic lupus erythematosus. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. Varying haplotypes of this gene cluster exist, such that individuals may have 1, 2, or 3 copies of this gene. In addition, this gene exists as a long form and a short form due to the presence or absence of a 6.4 kb endogenous HERV-K retrovirus in intron 9.
Purification Method:
Affinity purification
Gene ID:
721
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates, using C4c Rabbit pAb (CAB24351) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
