The C12orf65 Polyclonal Antibody (PAC047142) is a valuable tool for researchers studying the C12orf65 protein, which plays a role in cellular functions related to mitochondrial dynamics and energy metabolism. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the C12orf65 protein, this antibody enables precise detection and analysis in a variety of cell types, making it ideal for studies focused on mitochondrial biology and metabolic disorders.C12orf65, also known as FAM189B, is a mitochondrial protein that has been linked to processes such as mitochondrial fusion and fission, as well as the regulation of cellular energy production.
Dysregulation of C12orf65 has been associated with mitochondrial dysfunction and metabolic disorders, highlighting its importance in cellular homeostasis. Research on C12orf65 is essential for gaining insights into the mechanisms underlying these conditions and for developing potential therapeutic strategies targeting mitochondrial function.Overall, the C12orf65 Polyclonal Antibody offers researchers a reliable tool for investigating the functions and dysregulation of the C12orf65 protein in various cellular pathways, with potential implications for understanding and treating metabolic disorders and related diseases.
Antibody Name:
C12orf65 Antibody (PACO47142)
Antibody SKU:
PACO47142
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:10000, IHC:1:20-1:200
Species Reactivity:
Human
Immunogen:
Recombinant Human Probable peptide chain release factor C12orf65, mitochondrial protein (36-166AA)
Immunohistochemistry of paraffin-embedded human colon cancer using PACO47142 at dilution of 1:100.
Immunohistochemistry of paraffin-embedded human kidney tissue using PACO47142 at dilution of 1:100.
Background:
May act as a codon-independent translation release factor that has lost all stop codon specificity and directs the termination of translation in mitochondrion. May help rescuing stalled mitoribosomes during translation (By similarity).
C12orf65: May act as a codon-independent translation release factor that has lost all stop codon specificity and directs the termination of translation in mitochondrion. Defects in C12orf65 are the cause of combined oxidative phosphorylation deficiency type 7 (COXPD7). A mitochondrial disease resulting in encephalomyopathy. Clinical manifestations include psychomotor delay and regression, ataxia, optic atrophy, nystagmus and muscle atrophy and weakness. Belongs to the prokaryotic/mitochondrial release factor family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Translation; RNA-bindingChromosomal Location of Human Ortholog: 12q24.31Cellular Component: mitochondrionMolecular Function: translation release factor activityBiological Process: translational terminationDisease: Combined Oxidative Phosphorylation Deficiency 7
UniProt Protein Details:
NCBI Summary:
This nuclear gene encodes a mitochondrial matrix protein that appears to contribute to peptide chain termination in the mitochondrial translation machinery. Two different 1 bp deletions (resulting in the same premature stop codon)result in decreased mitochondrial translation, decreased levels of oxidative phosphorylation complexes and encepthalomyopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2010]
