The C10orf32 Antibody (CAB7276) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and is specifically validated for use in Western blot applications. By binding to the C10orf32 protein, researchers can effectively detect and analyze its expression in various cell types, making it an ideal choice for studies in oncology and immunology.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
C10orf32 Antibody
SKU:
CAB7276
Size:
20μL, 100μL
Reactivity:
Human, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
C10orf32
Positive Sample:
Rat brain
Cellular Localization:
Borc Complex.
Calculated MW:
12kDa
Observed MW:
18kDa
Part of BORC complex.
Purification Method
Affinity purification
Gene ID
119032
RRID
AB_2767817
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Rat brain, using C10orf32 Rabbit pAb (CAB7276) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of MCF-7 cells using C10orf32 Rabbit pAb (CAB7276). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
