The c-Fos Polyclonal Antibody (CAB2444) is a valuable tool for researchers studying the c-Fos protein, a key regulator of cellular processes such as cell growth, differentiation, and stress responses. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.The c-Fos protein is a transcription factor that plays a crucial role in controlling gene expression in response to various stimuli, including growth factors, hormones, and environmental stressors. By targeting the c-Fos protein, researchers can investigate its involvement in pathways related to cell proliferation, apoptosis, and oncogenesis.The versatility of the c-Fos Polyclonal Antibody makes it suitable for studies in a wide range of disciplines, including molecular biology, cancer research, and neuroscience.
Its ability to specifically bind to the c-Fos protein enables accurate detection and analysis in various cell types, providing valuable insights into the molecular mechanisms underlying cellular processes.Overall, the c-Fos Polyclonal Antibody is a reliable tool for researchers looking to explore the functions of the c-Fos protein in health and disease. Its application in experimental studies can contribute to a better understanding of the pathways regulated by c-Fos, potentially leading to the development of targeted therapies for cancer and other diseases influenced by c-Fos activity.
Product Name:
c-Fos Rabbit Polyclonal Antibody
SKU:
CAB2444
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-114 of human c-Fos (NP_005243.1).
The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death.
Purification Method:
Affinity purification
Gene ID:
2353
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of HeLa cells, using c-Fos antibody (CAB2444) at 1:1000 dilution.HeLa cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
