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c-Fos Rabbit Polyclonal Antibody (CAB2444)

SKU:
CAB2444
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
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  • Cell Biology Antibodies 8 Anti-c-Fos Antibody CAB2444
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  • Cell Biology Antibodies 8 Anti-c-Fos Antibody CAB2444
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:c-Fos Rabbit Polyclonal Antibody
SKU:CAB2444
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-114 of human c-Fos (NP_005243.1).
Sequence:MMFS GFNA DYEA SSSR CSSA SPAG DSLS YYHS PADS FSSM GSPV NAQD FCTD LAVS SANF IPTV TAIS TSPD LQWL VQPA LVSS VAPS QTRA PHPF GVPA PSAG AYSR AGVV KT
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:p55; AP-1; C-FOS; c-Fos
Positive Sample:HeLa
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Endoplasmic reticulum, Nucleus, cytosol.
Calculated MW:41kDa
Observed MW:55-60kDa

The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death.

Purification Method:Affinity purification
Gene ID:2353
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of HeLa cells, using c-Fos antibody (CAB2444) at 1:1000 dilution.HeLa cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

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