BYSL Rabbit Polyclonal Antibody (CAB11993)
- SKU:
- CAB11993
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
Product Name: | BYSL Rabbit Polyclonal Antibody |
SKU: | CAB11993 |
Size: | 20uL, 100uL |
Isotype: | IgG |
Host Species: | Rabbit |
Reactivity: | Human,Mouse,Rat |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 120-280 of human BYSL (NP_004044.3). |
Sequence: | HAEV VVDP EDER AIEM FMNK NPPA RRTL ADII MEKL TEKQ TEVE TVMS EVSG FPMP QLDP RVLE VYRG VREV LSKY RSGK LPKA FKII PALS NWEQ ILYV TEPE AWTA AAMY QATR IFAS NLKE RMAQ RFYN LVLL PRVR DDVA EYKR LNFH LYMA LKKA L |
Tested Applications: | WB IHC-P ELISA |
Recommended Dilution: | WB,1:500 - 1:2000 IHC-P,1:50 - 1:100 |
Synonyms: | Enp1; BYSTIN; BYSL |
Positive Sample: | HeLa,Jurkat,A-549,BxPC-3,Mouse testis,Mouse lung,Rat testis,Rat lung |
Conjugate: | Unconjugated |
Cellular Localization: | cytoplasm, cytosol, nucleolus, nucleoplasm |
Calculated MW: | 50kDa |
Observed MW: | 49kDa |
Bystin is expressed as a 2-kb major transcript and a 3.6-kb minor transcript in SNG-M cells and in human trophoblastic teratocarcinoma HT-H cells. Protein binding assays determined that bystin binds directly to trophinin and tastin, and that binding is enhanced when cytokeratins 8 and 18 are present. Immunocytochemistry of HT-H cells showed that bystin colocalizes with trophinin, tastin, and the cytokeratins, suggesting that these molecules form a complex in trophectoderm cells at the time of implantation. Using immunohistochemistry it was determined that trophinin and bystin are found in the placenta from the sixth week of pregnancy. Both proteins were localized in the cytoplasm of the syncytiotrophoblast in the chorionic villi and in endometrial decidual cells at the uteroplacental interface. After week 10, the levels of trophinin, tastin, and bystin decreased and then disappeared from placental villi.
Purification Method: | Affinity purification |
Gene ID: | 705 |
Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |