The BYSL Polyclonal Antibody (CAB11993) is a valuable tool for researchers studying the BYSL protein, which plays a key role in cell proliferation and mRNA processing. This antibody, generated in rabbits, specifically targets BYSL and is highly reactive with human samples, making it suitable for a variety of research applications such as Western blotting and immunohistochemistry.BYSL, also known as putative GTPase BYSTIN-like protein, is essential for ribosome biogenesis and cell growth. Dysregulation of BYSL expression has been linked to various diseases, including cancer and developmental disorders.
Therefore, understanding the function and regulation of BYSL is crucial for unraveling its role in normal cellular processes and disease pathology.Researchers in the fields of molecular biology, oncology, and developmental biology can benefit from the use of the BYSL Polyclonal Antibody for investigating the intricate mechanisms involving BYSL and its impact on cellular functions. Whether studying cell division, RNA metabolism, or disease progression, this antibody provides a reliable tool for detecting and analyzing BYSL protein levels in various experimental settings.
Product Name:
BYSL Rabbit Polyclonal Antibody
SKU:
CAB11993
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 120-280 of human BYSL (NP_004044.3).
Bystin is expressed as a 2-kb major transcript and a 3.6-kb minor transcript in SNG-M cells and in human trophoblastic teratocarcinoma HT-H cells. Protein binding assays determined that bystin binds directly to trophinin and tastin, and that binding is enhanced when cytokeratins 8 and 18 are present. Immunocytochemistry of HT-H cells showed that bystin colocalizes with trophinin, tastin, and the cytokeratins, suggesting that these molecules form a complex in trophectoderm cells at the time of implantation. Using immunohistochemistry it was determined that trophinin and bystin are found in the placenta from the sixth week of pregnancy. Both proteins were localized in the cytoplasm of the syncytiotrophoblast in the chorionic villi and in endometrial decidual cells at the uteroplacental interface. After week 10, the levels of trophinin, tastin, and bystin decreased and then disappeared from placental villi.
Purification Method:
Affinity purification
Gene ID:
705
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using BYSL Rabbit pAb (CAB11993) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
