The BUB3 Polyclonal Antibody (CAB6536) is a valuable tool for researchers studying BUB3, a key protein involved in cell division and mitosis. This antibody, generated in rabbits, exhibits strong reactivity with human samples and is validated for use in Western blotting applications. By binding specifically to the BUB3 protein, this antibody enables precise detection and analysis in a variety of cell types, making it an essential component for studies in cell biology and cancer research.
BUB3 is a crucial component of the mitotic spindle assembly checkpoint, ensuring accurate chromosome segregation during cell division. Dysregulation of BUB3 has been linked to chromosomal instability and tumorigenesis, highlighting its importance in cancer research. By investigating the function and regulation of BUB3, researchers can gain insights into potential therapeutic targets for cancer treatment and understand the mechanisms underlying cell division errors.
Product Name:
BUB3 Rabbit Polyclonal Antibody
SKU:
CAB6536
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-328 of human BUB3 (NP_004716.1).
This gene encodes a protein involved in spindle checkpoint function. The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Purification Method:
Affinity purification
Gene ID:
9184
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using BUB3 antibody (CAB6536) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (AbGn00021)._Exposure time: 90s.
