The BUB1B Polyclonal Antibody (CAB1775) is a powerful tool for researchers studying the BUB1B protein, a key player in cell division and chromosome segregation processes. This antibody, generated in rabbits, exhibits high reactivity with human samples and is specifically validated for use in Western blot applications. By binding to the BUB1B protein, researchers can easily detect and analyze its expression in a variety of cell types, making it ideal for studies in cell biology, oncology, and developmental biology.
BUB1B is essential for accurate chromosome segregation during mitosis and meiosis, ensuring the fidelity of genetic information transmission. Dysregulation of BUB1B has been implicated in various cancers and genetic disorders, highlighting its importance in maintaining genomic stability. By studying the function of BUB1B using this antibody, researchers can gain insights into its role in cell division and potentially identify new therapeutic targets for diseases associated with chromosomal instability.
Product Name:
BUB1B Rabbit Polyclonal Antibody
SKU:
CAB1775
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human BUB1B (NP_001202.4).
This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer.
Purification Method:
Affinity purification
Gene ID:
701
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using BUB1B antibody (CAB1775) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.
