The KO Validated BUB1 Rabbit Polyclonal Antibody (CAB18053) is a powerful tool for researchers studying the BUB1 protein, a key regulator of the cell cycle and mitosis. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards human samples, making it ideal for use in Western blot applications. By targeting the BUB1 protein, this antibody enables precise detection and analysis in various cell types, making it essential for studies in cell biology and cancer research.
BUB1, a critical component of the mitotic checkpoint complex, plays a vital role in ensuring accurate chromosome segregation during cell division. Dysregulation of BUB1 has been linked to chromosomal instability and tumorigenesis, highlighting its importance in cancer biology. Research on the functions and mechanisms of BUB1 is crucial for understanding its role in disease development and progression, offering insights into potential therapeutic targets for cancer treatment.
Product Name:
[KO Validated] BUB1 Rabbit Polyclonal Antibody
SKU:
CAB18053
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human BUB1 (NP_004327.1).
This gene encodes a serine/threonine-protein kinase that play a central role in mitosis. The encoded protein functions in part by phosphorylating members of the mitotic checkpoint complex and activating the spindle checkpoint. This protein also plays a role in inhibiting the activation of the anaphase promoting complex/cyclosome. This protein may also function in the DNA damage response. Mutations in this gene have been associated with aneuploidy and several forms of cancer. Alternate splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
699
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and BUB1 knockout (KO) HeLa cells, using [KO Validated] BUB1 Rabbit pAb (CAB18053) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1min.
