The BTRC Polyclonal Antibody (CAB18232) is a powerful tool for researchers studying BTRC, a protein involved in the regulation of key cellular processes such as protein degradation and cell cycle control. This rabbit-derived antibody is highly specific to human samples and has been validated for use in Western blot applications. By binding to the BTRC protein, it allows for accurate detection and analysis in a variety of cell types, making it a valuable asset for studies in molecular biology and cancer research.
BTRC, also known as beta-transducin repeat-containing protein, plays a crucial role in directing the breakdown of proteins that control cell growth and division. Dysregulation of BTRC has been linked to various diseases, including cancer and neurodegenerative disorders, making it an important target for therapeutic development. By understanding the function of BTRC, researchers can uncover new insights into disease mechanisms and potentially identify novel treatment strategies.
Product Name:
BTRC Rabbit Polyclonal Antibody
SKU:
CAB18232
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human β-TrCP/BTRC (NP_378663.1).
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class; in addition to an F-box, this protein contains multiple WD-40 repeats. The encoded protein mediates degradation of CD4 via its interaction with HIV-1 Vpu. It has also been shown to ubiquitinate phosphorylated NFKBIA (nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha), targeting it for degradation and thus activating nuclear factor kappa-B. Alternatively spliced transcript variants have been described. A related pseudogene exists in chromosome 6.
Purification Method:
Affinity purification
Gene ID:
8945
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using β-TrCP/BTRC Rabbit pAb (CAB18232) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
