The BTNL2 Polyclonal Antibody (PAC04487) is specifically designed for research involving BTNL2, a cell surface molecule with important immunoregulatory functions. This antibody, produced in rabbits, shows high reactivity with human samples and has been validated for use in Western blot applications. By binding to the BTNL2 protein, this antibody allows for easy detection and analysis in various cell types, making it an ideal tool for studies in immunology and cancer research.
BTNL2, also known as Butyrophilin-like 2, is involved in immune regulation and has been implicated in the modulation of immune responses. Its role in immune homeostasis and potential impact on diseases such as cancer, autoimmune disorders, and chronic inflammatory conditions make it a promising target for further research. Understanding the function of BTNL2 is essential for developing therapies that can either enhance or suppress immune responses in these disease contexts.
BTNL2; Butyrophilin-like protein 2 antibody; BTL-II antibody
Species Reactivity:
Human
Immunogen:
Recombinant Human Butyrophilin-like protein 2 protein (246-455AA)
Immunogen Species:
Homo sapiens (Human)
Clonality:
Polyclonal
Purification Method:
Antigen Affinity Purified
Conjugate:
Non-conjugated
Form:
Liquid
Storage:
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time:
Basically, we can dispatch the products out in 7-10 working days after receiving your orders. Delivery time may differ depending on the purchasing method or location. Please consult your local distributors for specific delivery times.
Tested Applications:
ELISAIHCIF
Recommended Dilution:
Application
Recommended Dilution
IHC
1:20-1:200
IF
1:20-1:200
Description:
Assay Genie uses the peptide mapping within amino acids 246-455 of the recombinant human BTNL2 to immunize rabbits to yield the anti-BTNL2 antibody. It is a polyclonal antibody and occurs as an unconjugated IgG. And it underwent antigen affinity purified. This BTNL2 antibody can detect with human and mouse BTNL2 protein, an MHC class II gene-linked butyrophilin-like molecule, with the human autoimmune diseases sarcoidosis and myositis. And it has been verified to recognize the BTNL2 protein in ELISA, WB, and IHC applications.
Function:
Negative regulator of T-cell proliferation.
Gene References into Functions:
Host-genotype analysis revealed an association of the rs2076530 (BTNL2) risk allele with a decrease in bacterial burden in sarcoidosis. PMID: 29242257
HLA-DPB1*05:01 gene was associated with the geographical region of PV and the BTNL2 gene was significantly associated with family history and age of onset of PV. In conclusion, the HLA-DPB1*05:01 and BTNL2 genes might be responsible for the complicacy of clinical features. PMID: 28581127
The sarcoidosis risk variant BTNL2 c.1078G.A (rs2076530) was associated with disease in all OFG cases (P (1/4) 0.013; OR (1/4) 1.33; 95% CI, 1.06-1.67) and had a similar OR (1.56) and the same direction of effect as seen in sarcoidosis. However, no association was seen for the subphenotypes of OFG only or OFG+CD. PMID: 27306066
Despite a significant difference in BTNL2 polymorphism between sarcoid patients and controls, there was no such difference between familial and sporadic sarcoidosis cases and no correlation between BTNL2 polymorphism and disease severity or outcome. Thus, BTNL2 difference cannot be considered as a key marker for disease classification or patient management. PMID: 27914482
Two new signals were observed at genome-wide significance (P < 5 x 10-8), namely, rs7216064 (17q24.3, BPTF), for overall lung adenocarcinoma risk, and rs3817963 (6p21.3, BTNL2) which is specific to cases with EGFR mutations. In further sub-analyses by EGFR status, rs9387478 (ROS1/DCBLD1) and rs2179920 (HLA-DPB1) showed stronger estimated associations in EGFR-positive compared to EGFR-negative cases PMID: 28025329
BTNL2 variant is associated with psoriasis. PMID: 27213287
Butyrophilin-like 2, expressed at various levels by UM cells and macrophages, might interfere with the immune control of the tumor. Butyrophilin-like 2 variants showed highly variable frequencies among ethnically related cohorts. There was no enrichment of BTNL2 variants in patients with UM compared with control patients. PMID: 27532663
BTNL2 G16071A gene polymorphism may as a likelihood factor contributed to granulomatous disease susceptibility, especially increasing the sarcoidosis susceptibility. In addition, the polymorphism may be greatly associated with likelihood of granulomatous diseases among Caucasians. PMID: 27472712
BTNL2 may have an inhibitory effect on FOXP3(+) T cell proliferation, especially in patients homozygous for the risk alleles. PMID: 26679868
genetic mutations within or around BTNL2 (rs3763313, rs9268494, rs9268492 and rs9268402) could alter susceptibility to grade IV of dilated cardiomyopathy in a Chinese population PMID: 26617759
Results confirm the association of BTNL2 rs2076530SNP with the susceptibility to develop sarcoidosis, but not with an increased risk of cancer in these patients. PMID: 25078641
BTNL2 rs2076530 polymorphism contributes to the risk of sarcoidosis PMID: 25849037
Two independent loci near BTNL2 (rs9461741) and HLA-B (rs2922994) in the HLA region significantly associated with Marginal Zone B-Cell Lymphoma risk. PMID: 25569183
The association of the rare variant p.G454C in BTNL2 reached genome-wide significance, and was independent of the known common risk variants for IBD in the HLA region in both a conditional and haplotype analysis PMID: 25671699
These findings suggest that polymorphisms in the BTNL2 gene might play a vital role in determining the outcome of the immune response to hepatitis B vaccination. PMID: 24664813
No variation except in the 329-bp region containing the BTNL2 rs2076530 polymorphism was found in Turkish patients with sarcoidosis. PMID: 25551927
Rare BTNL2 variants play a role in susceptibility to both familial and sporadic prostate cancer. Results implicate BTNL2 as a novel prostate cancer susceptibility gene. PMID: 23833122
the BTNL2 splice site polymorphism (A variant of rs2076530) shows association with an increased risk for persistent sarcoidosis PMID: 23904553
sequenced single-nucleotide variants in an association study of 432 cases and 432 controls showed that 12 non-synonymous single-nucleotide polymorphisms (SNPs) in BTNL2 were significantly associated with RA PMID: 23364395
Results show that single nucleotide polymorphism associated with BTNL2 gene is a risk factor for predisposition pulmonary sarcoidosis PMID: 23017494
Upon stratification analysis in search for a synergistic effect in sarcoidosis given the extensive linkage disequilibrium between BTNL2 rs2076530_A and HLA-DRB1*08:03; the risk-bearing allele of these two loci interact negatively. PMID: 22991420
We unravel the role of unexplored immunologically important genes, BAT1 and BTNL2, and the haplotypes of the significantly associated SNPs therein, to understand susceptibility to the disease, leprosy and its differential severity. PMID: 22071774
The rs10947262 and rs7775228 variants are not associated with risk of knee osteoarthritis in European descent populations. PMID: 21853121
The BTNL2 A allele variant occurs with a high frequency in Danish patients with sarcoidosis PMID: 21410903
The presence of a BTNL2G16071A variant allele almost doubles the risk of progressing to persistent pulmonary sarcoidosis in addition to increasing the risk of developing sarcoidosis. PMID: 21256912
preliminary data suggest that BTNL2 polymorphism may be associated with susceptibility to Kawasaki disease and coronary artery lesions in Taiwanese children PMID: 19882345
This genetic study reveals a significant association between the rs3763313, rs9268494, rs9268492 SNPs in the BTNL2 gene and tuberculosis. PMID: 20176143
the absence of a membrane anchored BTNL2 protein may increase genetic susceptibility to sarcoidosis and familial occurrence of the disease. PMID: 20560297
Sarcoidosis is associated with a truncating splice site mutation in BTNL2 PMID: 15735647
BNTL2 associates with sarcoidosis in both White Americans and African-Americans, with risk effects in Whites independent of HLA-DRB1 associations, and negative interactions between BRNL2 and HLA classII in African-Americans. PMID: 16080124
Association of the truncating splice site mutation in BTNL2 with multiple sclerosis is secondary to HLA-DRB1*15. PMID: 16321988
resiults show that BTNL2 rs2076530 polymorphism is associated with type 1 diabetes, rheumatoid arthritis, and systemic lupus erythematosus because of its strong linkage disequalibrium with predisposing HLA DQB1-DRB1 haplotypes in Caucasian populations PMID: 16690410
RAGE and its ligands with sarcoidosis and suggest that an intrinsic genetic factor could be in part involved in its expression. In Italian patients, the -374 T/A polymorphism seems to be significantly associated with this disease. PMID: 17170388
Structural analysis of BTNL2 shows a molecule with an extracellular region containing two sets of two Ig domains, a transmembrane region, and a previously unreported cytoplasmic tail PMID: 17237401
Coding regions of BTNL2 were sequenced to detect known and novel polymorphisms and genotyped 18 SNPs in 432 pulmonary tuberculosis cases and 482 controls. PMID: 17347014
a major effect of the BTNL2 rs2076530 (G --> A) SNP in Crohn's disease and tuberculosis was excluded; there was an association with susceptibility to leprosy (P=0.04), however, this is most likely due to linkage disequilibrium with HLA-DR PMID: 17493147
Does not contribute to susceptibility to Crohn's disease in Japan but is associated with Japanese ulcerative colitis because of strong linkage disequilibrium with HLA-DRB1*1502. PMID: 17610417
Some haplotypes are associated with non-Lofgren sarcoidosis. PMID: 17661910
Both DRB1*13 and BTNL2 rs3117099TT homozygosity are associated with chronic beryllium disease [CBD] in *Glu69-negative subjects, while DPB1*Glu69 is associated with CBD and Be sensitivity compared with Be exposed PMID: 17927685
BTNL2 gene might be one of the candidate genes that is responsible for the pathogenesis of Dermatophagoides farinae (Der f)-specific IgE responsiveness. PMID: 19050377
Genome-wide significant evidence for association was found in a region spanning BTNL2 to HLA-DQB1 on chromosome 6p21 for ulcerative colitis. PMID: 19122664
The CNV_ID 507 was tested for association in a cohort of 89 sarcoidosis patients and 89 matched controls, but our results indicated that CNV_ID 507 does not affect the genomic structure of BTLN2 as previously described PMID: 19140834
Single-nucleotide polymorphism in the BTNL2 gene is associated with ulcerative colitis. PMID: 19659809
Subcellular Location:
Membrane; Single-pass type II membrane protein. Note=Isoform 2 is present in the nuclear, vesicle and plasma membranes, isoform 3 is found in cytoplasmic vesicle structures and is not membrane bound.
Protein Families:
Immunoglobulin superfamily, BTN/MOG family
Tissue Specificity:
Expressed in brain, heart, kidney, liver, pancreas, ovary, leukocyte, small intestine, testis and thymus.
IHC diluted at 1:50 and staining in paraffin-embedded human Small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
IHC diluted at 1:50 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence staining of HepG2 cell with PACO44487 at 1:40, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
