The BTN3A3 Polyclonal Antibody (CAB14269) is a vital tool for researchers studying the BTN3A3 protein, a member of the butyrophilin family known for its involvement in immune regulation and modulation of T cell responses. Produced using rabbits as a host, this antibody is highly specific to human samples and has been rigorously validated for Western blot applications. By targeting the BTN3A3 protein, this antibody allows for precise detection and analysis in a variety of cell types, making it an invaluable asset for investigations in immunology and cancer research.
BTN3A3 is a key player in immune system functioning, with its role in T cell activation and immune response regulation making it a promising target for studying diseases such as cancer, autoimmune disorders, and inflammatory conditions. Understanding the mechanisms by which BTN3A3 operates is crucial for the development of novel therapeutic strategies aimed at manipulating immune responses in these disease contexts. Get your hands on the BTN3A3 Polyclonal Antibody to advance your research in this important area of study.
Product Name:
BTN3A3 Rabbit Polyclonal Antibody
SKU:
CAB14269
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 510-584 of human BTN3A3 (NP_008925.1).
Sequence:
TICP IPKE VESS PDPD LVPD HSLE TPLT PGLA NESG EPQA EVTS LLLP AHPG AEVS PSAT TNQN HKLQ ARTE ALY
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
BTF3; BTN3.3; BTN3A3
Positive Sample:
Raji,Jurkat,Mouse spleen,Mouse liver,Rat spleen
Conjugate:
Unconjugated
Cellular Localization:
Cell membrane, Single-pass type I membrane protein.
Calculated MW:
65kDa
Observed MW:
65kDa
The butyrophilin (BTN) genes are a group of major histocompatibility complex (MHC)-associated genes that encode type I membrane proteins with 2 extracellular immunoglobulin (Ig) domains and an intracellular B30.2 (PRYSPRY) domain. Three subfamilies of human BTN genes are located in the MHC class I region: the single-copy BTN1A1 gene (MIM 601610) and the BTN2 (e.g., BTN2A1; MIM 613590) and BTN3 (e.g., BNT3A3) genes, which have undergone tandem duplication, resulting in 3 copies of each (summary by Smith et al., 2010 [PubMed 20208008]).
Purification Method:
Affinity purification
Gene ID:
10384
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using BTN3A3 Rabbit pAb (CAB14269) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
