The Brox Antibody (PACO61005) is a polyclonal antibody designed for research involving Brox, a protein known to play a critical role in autophagy and cellular homeostasis. This antibody, raised in rabbits, is highly reactive with human samples and is validated for use in various applications including Western blot, immunofluorescence, and immunohistochemistry.Brox, a key player in autophagy, is involved in the process of cellular self-degradation and recycling to maintain cellular health and function. Dysregulation of autophagy has been linked to various diseases including cancer, neurodegenerative disorders, and metabolic diseases.
By studying the role of Brox in autophagy, researchers can gain valuable insights into disease mechanisms and potential therapeutic targets.With its high reactivity and reliability in various experimental techniques, the Brox Antibody (PACO61005) is an essential tool for researchers studying autophagy, cellular homeostasis, and related disease pathways. Its ability to specifically bind to the Brox protein enables precise detection and analysis in different cell types, making it a valuable asset in advancing our understanding of cellular processes and disease mechanisms.
IHC image of PACO61005 diluted at 1:200 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with PACO61005 at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western Blot. Positive WB detected in: K562 whole cell lysate. All lanes: BROX antibody at 3µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 47, 43 kDa. Observed band size: 47 kDa.
Background:
extracellular exosome
Synonyms:
BRO1 domain-containing protein BROX (BRO1 domain- and CAAX motif-containing protein), BROX, BROFTI C1orf58
UniProt Protein Function:
BROX: Belongs to the BROX family.Protein type: Unknown functionChromosomal Location of Human Ortholog: 1q41Cellular Component: membrane
