BRI1 Polyclonal Antibody (CAB21279)
- SKU:
- CAB21279
- Product Type:
- Antibody
- Antibody Type:
- Polyclonal Antibody
- Reactivity:
- Plant - Arabidopsis
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- WB
Description
Product Name: | BRI1 Polyclonal Antibody |
SKU: | CAB21279 |
Size: | 20uL, 100uL |
Isotype: | IgG |
Host Species: | Rabbit |
Reactivity: | Arabidopsis thaliana |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 24-280 of arabidopsis thaliana BRI1 (NP_195650.1). |
Sequence: | FQAS PSQS LYRE IHQL ISFK DVLP DKNL LPDW SSNK NPCT FDGV TCRD DKVT SIDL SSKP LNVG FSAV SSSL LSLT GLES LFLS NSHI NGSV SGFK CSAS LTSL DLSR NSLS GPVT TLTS LGSC SGLK FLNV SSNT LDFP GKVS GGLK LNSL EVLD LSAN SISG ANVV GWVL SDGC GELK HLAI SGNK ISGD VDVS RCVN LEFL DVSS NNFS TGIP FLGD CSAL QHLD ISGN KLSG DFSR AIST CTEL KLLN ISSN Q |
Tested Applications: | WB ELISA |
Recommended Dilution: | WB,1:100 - 1:500 |
Synonyms: | ATBRI1; BIN1; BR INSENSITIVE 1; BRASSINOSTEROID INSENSITIVE 1; CABBAGE 2; CBB2; DWARF 2; DWF2; F23K16.30; F23K16_30; BRI1 |
Positive Sample: | Before inflorescence,Rosette leaf |
Conjugate: | Unconjugated |
Cellular Localization: | endosome, plasma membrane |
Calculated MW: | 131kDa |
Observed MW: | 131kDa |
Encodes a plasma membrane localized leucine-rich repeat receptor kinase involved in brassinosteroid signal transduction. BRI1 ligand is brassinolide which binds at the extracellular domain. Binding results in phosphorylation of the kinase domain which activates the BRI1 protein leading to BR responses. Residue T-1049 and either S-1044 or T-1045 were essential for kinase function in vitro and normal BRI1 signaling in planta. The structure of BRI1 ligand-binding domain has been determined at 2.5A resolution. Although BAK1 and BRI1 alone localize in the plasma membrane, when BAK1 and BRI1 are coexpressed, the heterodimer BAK1/BRI1 they form is localized in the endosome. BRI1 appears to be involved in the autonomous pathway that regulates the transition to flowering, primarily through its effects on FLC expression levels, as uncovered by double mutant analyses. This most likely occurs as a result of BRI1-dependent effects on histone acetylation, but not histone triMeH3K4 methylation, at the FLC locus.
Purification Method: | Affinity purification |
Gene ID: | 830095 |
Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3. |