The BRI1 Polyclonal Antibody is a valuable tool for researchers studying the role of BRI1, a key receptor protein involved in plant growth and development. This antibody, produced in rabbits, shows high reactivity with plant samples and is validated for use in various experimental techniques, including Western blot analysis. By binding specifically to the BRI1 protein, this antibody enables precise detection and analysis, making it ideal for investigations in plant biology and agriculture.BRI1, also known as brassinosteroid-insensitive 1 receptor kinase, plays a crucial role in brassinosteroid signaling pathways, which control various aspects of plant growth and stress responses.
Understanding the function of BRI1 is essential for advancing research in crop improvement, stress tolerance, and plant development. By studying the activity of BRI1, researchers can gain insights into how plants respond to environmental cues and optimize growth under different conditions.Overall, the BRI1 Polyclonal Antibody provides a powerful tool for exploring the functions of BRI1 in plant biology, offering researchers the ability to delve deeper into the intricate mechanisms that govern plant growth and development. Its versatility and specificity make it an indispensable asset for studies aiming to enhance agricultural productivity and sustainability.
Product Name:
BRI1 Polyclonal Antibody
SKU:
CAB21279
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Arabidopsis thaliana
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 24-280 of arabidopsis thaliana BRI1 (NP_195650.1).
Encodes a plasma membrane localized leucine-rich repeat receptor kinase involved in brassinosteroid signal transduction. BRI1 ligand is brassinolide which binds at the extracellular domain. Binding results in phosphorylation of the kinase domain which activates the BRI1 protein leading to BR responses. Residue T-1049 and either S-1044 or T-1045 were essential for kinase function in vitro and normal BRI1 signaling in planta. The structure of BRI1 ligand-binding domain has been determined at 2.5A resolution. Although BAK1 and BRI1 alone localize in the plasma membrane, when BAK1 and BRI1 are coexpressed, the heterodimer BAK1/BRI1 they form is localized in the endosome. BRI1 appears to be involved in the autonomous pathway that regulates the transition to flowering, primarily through its effects on FLC expression levels, as uncovered by double mutant analyses. This most likely occurs as a result of BRI1-dependent effects on histone acetylation, but not histone triMeH3K4 methylation, at the FLC locus.
Purification Method:
Affinity purification
Gene ID:
830095
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various tissues from Arabidopsis thaliana, using BRI1 Rabbit pAb (CAB21279) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
