The BRF2 Polyclonal Antibody (CAB8782) is an essential tool for researchers studying BRF2, a key transcription factor involved in regulating RNA polymerase III (Pol III) activity. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot experiments.BRF2 is a critical component of the Pol III transcription complex, which is responsible for transcribing small non-coding RNAs essential for cell growth and proliferation. Dysregulation of Pol III activity has been implicated in various diseases, including cancer and neurological disorders.
By targeting BRF2, researchers can gain insights into the mechanisms underlying these diseases and potential therapeutic opportunities.The BRF2 Polyclonal Antibody enables precise detection and analysis of BRF2 protein levels in different cell types, making it an invaluable tool for studies in molecular biology and cancer research. With its specificity and sensitivity, this antibody offers researchers the ability to unravel the intricate functions of BRF2 in cellular processes, paving the way for new discoveries and advancements in the field.
Product Name:
BRF2 Rabbit Polyclonal Antibody
SKU:
CAB8782
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 180-419 of human BRF2 (NP_060780.2).
This gene encodes one of the multiple subunits of the RNA polymerase III transcription factor complex required for transcription of genes with promoter elements upstream of the initiation site. The product of this gene, a TFIIB-like factor, is directly recruited to the TATA-box of polymerase III small nuclear RNA gene promoters through its interaction with the TATA-binding protein.
Purification Method:
Affinity purification
Gene ID:
55290
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using BRF2 Rabbit pAb (CAB8782) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
