The BRD4 Rabbit Monoclonal Antibody is a key tool for researchers studying BRD4, a protein involved in gene transcription regulation and cell cycle control. This antibody, produced from rabbit cells, shows high specificity towards human samples and has been validated for use in Western blotting applications. By targeting the BRD4 protein, researchers can easily detect and analyze its expression in various cell types, making it ideal for studies in oncology, epigenetics, and gene regulation.
BRD4 is a crucial player in several cellular processes, including cell growth, differentiation, and survival. It is also known to play a role in cancer development and progression, making it a promising target for cancer therapy research. By understanding the function and regulation of BRD4, researchers can potentially develop novel treatments for cancer and other diseases related to abnormal gene expression and cell proliferation.
Product Name:
BRD4 Rabbit Monoclonal Antibody
SKU:
CAB12677
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human BRD4 (O60885).
The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described.
Purification Method:
Affinity purification
Gene ID:
23476
Clone Number:
ARC0699
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using BRD4 Rabbit mAb (CAB12677) at 1：1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
