The BRCA2 Polyclonal Antibody (CAB2435) is a valuable tool for researchers studying the BRCA2 gene, known for its roles in DNA repair and breast cancer susceptibility. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards human samples, making it ideal for use in Western blot applications. By targeting the BRCA2 protein, this antibody enables researchers to detect and analyze BRCA2 expression in a variety of cell types, facilitating studies in oncology and genetics.
BRCA2, a tumor suppressor gene, is integral in maintaining genome stability and preventing the formation of cancerous cells. Mutations in the BRCA2 gene are associated with an increased risk of developing breast and ovarian cancers. Investigating the function and regulation of BRCA2 with this polyclonal antibody can provide valuable insights into the mechanisms underlying these diseases, ultimately aiding in the development of targeted therapies and personalized treatment strategies.
Product Name:
BRCA2 Rabbit Polyclonal Antibody
SKU:
CAB2435
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1400-1600 of BRCA2 (NP_000050.3).
Inherited mutations in BRCA1 and this gene, BRCA2, confer increased lifetime risk of developing breast or ovarian cancer. Both BRCA1 and BRCA2 are involved in maintenance of genome stability, specifically the homologous recombination pathway for double-strand DNA repair. The largest exon in both genes is exon 11, which harbors the most important and frequent mutations in breast cancer patients. The BRCA2 gene was found on chromosome 13q12.3 in human. The BRCA2 protein contains several copies of a 70 aa motif called the BRC motif, and these motifs mediate binding to the RAD51 recombinase which functions in DNA repair. BRCA2 is considered a tumor suppressor gene, as tumors with BRCA2 mutations generally exhibit loss of heterozygosity (LOH) of the wild-type allele.
Purification Method:
Affinity purification
Gene ID:
675
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse liver, using BRCA2 Rabbit pAb (CAB2435) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
