The BRCA1 Polyclonal Antibody (CAB11549) is a valuable tool for researchers studying the protein encoded by the BRCA1 gene, which is known for its role in DNA repair and maintenance of genomic stability. This antibody, raised in rabbits, is highly specific to human samples and has been validated for use in Western blotting applications.BRCA1 is a tumor suppressor gene that, when mutated, can increase the risk of developing breast and ovarian cancer. By using this antibody to detect and analyze the BRCA1 protein in various cell types, researchers can gain more insight into the molecular mechanisms underlying cancer development and potentially identify new therapeutic targets.
The BRCA1 Polyclonal Antibody is an essential tool for advancing research in cancer biology and personalized medicine, as it provides a reliable method for studying the expression and function of the BRCA1 gene in both healthy and diseased cells. Its high specificity and sensitivity make it an ideal choice for scientists working in the fields of oncology, genetics, and molecular biology.
Product Name:
BRCA1 Rabbit Polyclonal Antibody
SKU:
CAB11549
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 901-1000 of human BRCA1 (NP_009225.1).
This gene encodes a 190 kD nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The BRCA1 gene contains 22 exons spanning about 110 kb of DNA. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified.
Purification Method:
Affinity purification
Gene ID:
672
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Immunohistochemistry analysis of paraffin-embedded human lung cancer using BRCA1 Rabbit pAb (CAB11549) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
