Anti-BRCA1 Antibody (CAB11034)
- SKU:
- CAB11034
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Frequently bought together:
Description
Anti-BRCA1 Antibody (CAB11034)
The BRCA1 Polyclonal Antibody (CAB11034) is a vital tool for research focused on the BRCA1 protein, a tumor suppressor gene associated with hereditary breast and ovarian cancer. This antibody, produced in rabbits, exhibits high reactivity towards human samples and is specifically validated for use in Western blot applications. By targeting the BRCA1 protein, this antibody allows for the detailed detection and analysis of BRCA1 expression in a variety of cell types, making it an essential tool for studies in oncology and genetics.
BRCA1, known for its role in DNA repair and cell cycle regulation, is crucial for maintaining genomic stability and preventing the development of cancer. Mutations in the BRCA1 gene have been linked to an increased risk of certain cancers, making it a key focus of research in the field of cancer biology. Understanding the function and regulation of BRCA1 is essential for developing targeted therapies and diagnostic tools for individuals at risk of hereditary cancer syndromes.
| Antibody Name: | Anti-BRCA1 Antibody |
| Antibody SKU: | CAB11034 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 400-699 of human BRCA1 (NP_009230.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HL-60, HeLa, K-562, Mouse liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 400-699 of human BRCA1 (NP_009230.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LDDR WYMH SCSG SLQN RNYP SQEE LIKV VDVE EQQL EESG PHDL TETS YLPR QDLE GTPY LESG ISLF SDDP ESDP SEDR APES ARVG NIPS STSA LKVP QLKV AESA QSPA AAHT TDTA GYNA MEES VSRE KPEL TAST ERVN KRMS MVVS GLTP EEFM LVYK FARK HHIT LTNL ITEE TTHV VMKT DAEF VCER TLKY FLGI AGGK WVVS YFWV TQSI KERK MLNE HDFE VRGD VVNG RNHQ GPKR ARES QDRK IFRG LEIC CYGP FTNM PTGC PPNC GCAA RCLD RGQW LPCN WADV |
| Gene ID: | 672 |
| Uniprot: | P38398 |
| Cellular Location: | Chromosome, Cytoplasm, Cytoplasm, Nucleus |
| Calculated MW: | 7kDa/78-85kDa/202-210kDa |
| Observed MW: | 280kDa |
| Synonyms: | BRCA1, BRCAI, BRCC1, BROVCA1, FANCS, IRIS, PNCA4, PPP1R53, PSCP, RNF53 |
| Background: | This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified. |
| UniProt Protein Function: | E3 ubiquitin-protein ligase that specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and plays a central role in DNA repair by facilitating cellular responses to DNA damage. It is unclear whether it also mediates the formation of other types of polyubiquitin chains. The E3 ubiquitin-protein ligase activity is required for its tumor suppressor function. The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Regulates centrosomal microtubule nucleation. Required for normal cell cycle progression from G2 to mitosis. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator. Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation. Contributes to homologous recombination repair (HRR) via its direct interaction with PALB2, fine-tunes recombinational repair partly through its modulatory role in the PALB2-dependent loading of BRCA2-RAD51 repair machinery at DNA breaks. Component of the BRCA1-RBBP8 complex which regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage via BRCA1-mediated ubiquitination of RBBP8. Acts as a transcriptional activator (PubMed:20160719). |
| NCBI Summary: | This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified. [provided by RefSeq, May 2009] |
| UniProt Code: | P38398 |
| NCBI GenInfo Identifier: | 728984 |
| NCBI Gene ID: | 672 |
| NCBI Accession: | P38398.2 |
| UniProt Secondary Accession: | P38398,O15129, Q1RMC1, Q3LRJ0, Q3LRJ6, Q6IN79, Q7KYU9 E9PFZ0, |
| UniProt Related Accession: | P38398 |
| Molecular Weight: | 208kDa |
| NCBI Full Name: | Breast cancer type 1 susceptibility protein |
| NCBI Synonym Full Names: | BRCA1, DNA repair associated |
| NCBI Official Symbol: | BRCA1Â Â |
| NCBI Official Synonym Symbols: | IRIS; PSCP; BRCAI; BRCC1; FANCS; PNCA4; RNF53; BROVCA1; PPP1R53Â Â |
| NCBI Protein Information: | breast cancer type 1 susceptibility protein |
| UniProt Protein Name: | Breast cancer type 1 susceptibility protein |
| UniProt Synonym Protein Names: | RING finger protein 53; RING-type E3 ubiquitin transferase BRCA1Curated |
| Protein Family: | BRCA1-A complex |
| UniProt Gene Name: | BRCA1Â Â |
 | Western blot analysis of extracts of various cell lines, using BRCA1 antibody (CAB11034) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s. |
 | Immunofluorescence analysis of C6 cells using BRCA1 antibody (CAB11034) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2 OS cells using BRCA1 antibody (CAB11034) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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