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B-Raf Rabbit Polyclonal Antibody (CAB2988)

SKU:
CAB2988
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
€139 - €419
Frequently bought together:

Description

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B-Raf Rabbit Polyclonal Antibody (CAB2988)

The BRAF Polyclonal Antibody (CAB2988) is a valuable tool for researchers studying BRAF, a protein that plays a key role in cell signaling pathways and is commonly mutated in various cancers. This antibody, produced in rabbits, exhibits high reactivity with BRAF in human samples and has been validated for use in Western blot applications. By specifically binding to the BRAF protein, researchers can detect and analyze BRAF expression in a variety of cell types, making it a crucial tool for investigations in oncology and molecular biology.BRAF is a critical component of the MAPK/ERK signaling pathway, which regulates cell growth, proliferation, and differentiation. Mutations in the BRAF gene can lead to uncontrolled cell growth and contribute to the development of various malignancies.

Understanding the role of BRAF in cancer progression is essential for the development of targeted therapies that can effectively inhibit BRAF pathway activity and improve patient outcomes.In summary, the BRAF Polyclonal Antibody (CAB2988) is a reliable reagent for researchers seeking to study the function of BRAF in cancer and other diseases. Its specificity and sensitivity make it an ideal choice for investigating the molecular mechanisms underlying BRAF signaling and developing novel therapeutic strategies to target this important protein.

Product Name:B-Raf Rabbit Polyclonal Antibody
SKU:CAB2988
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 153-313 of human B-Raf (NP_004324.2).
Sequence:QKPI VRVF LPNK QRTV VPAR CGVT VRDS LKKA LMMR GLIP ECCA VYRI QDGE KKPI GWDT DISW LTGE ELHV EVLE NVPL TTHN FVRK TFFT LAFC DFCR KLLF QGFR CQTC GYKF HQRC STEV PLMC VNYD QLDL LFVS KFFE HHPI PQEE ASLA ETAL T
Tested Applications:WB ELISA
Recommended Dilution:WB,1:100 - 1:500
Synonyms:NS7; B-raf; BRAF1; RAFB1; B-RAF1; BRAF-1; B-Raf
Positive Sample:COS-7,WCH
Conjugate:Unconjugated
Cellular Localization:Cell membrane, Cytoplasm, Nucleus.
Calculated MW:84kDa
Observed MW:87kDa

This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome.

Purification Method:Affinity purification
Gene ID:673
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of various cell lines, using B-Raf antibody (CAB2988) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.

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