B-Raf Rabbit Polyclonal Antibody (CAB15033)
- SKU:
- CAB15033
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
Product Name: | B-Raf Rabbit Polyclonal Antibody |
SKU: | CAB15033 |
Size: | 20uL, 100uL |
Isotype: | IgG |
Host Species: | Rabbit |
Reactivity: | Human,Mouse,Rat |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 350-449 of human B-Raf (NP_004324.2). |
Sequence: | DEDH RNQF GQRD RSSS APNV HINT IEPV NIDD LIRD QGFR GDGG STTG LSAT PPAS LPGS LTNV KALQ KSPG PQRE RKSS SSSE DRNR MKTL GRRD SSDD |
Tested Applications: | WB IHC-P ELISA |
Recommended Dilution: | WB,1:500 - 1:2000 IHC-P,1:50 - 1:200 |
Synonyms: | NS7; B-raf; BRAF1; RAFB1; B-RAF1; BRAF-1; B-Raf |
Positive Sample: | LO2,U-87MG,HeLa,Jurkat |
Conjugate: | Unconjugated |
Cellular Localization: | Cell membrane, Cytoplasm, Nucleus. |
Calculated MW: | 84kDa |
Observed MW: | 105kDa |
This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome.
Purification Method: | Affinity purification |
Gene ID: | 673 |
Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. |