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BP (Benzylpenicillin) ELISA Kit (FSES0067)

SKU:
FSES0067
Product Type:
ELISA Kit
Applications:
ELISA
Research Area:
Food Science
€599
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Description

Technical Manual

BP (Benzylpenicillin) ELISA Kit

Kit Name: BP (Benzylpenicillin) ELISA Kit
Kit Size: 96 Assays
Test Principle: This kit uses Competitive-ELISA as the method. It can detect BP (Benzylpenicillin) in samples, such as tissue, etc. This kit is composed of ELISA Microtiter plate, HRP conjugate, antibody working solution, standard and other supplementary reagents. The microtiter plate provided in this kit has been pre-coated with coupled antigen. During the reaction, BP in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti- BP antibody Then Horseradish Peroxidase (HRP) conjugate is added to each microtiter plate well, and TMB substrate is added for color development. There is a negative correlation between the OD value of samples and the concentration of BP. The concentration of BP in the samples can be calculated by comparing the OD of the samples to the standard curve.
Sensitivity: 0.3 ppb (ng/mL)
Reaction Mode: 25°C, 30 min~ 30 min ~ 15 min
Detection Limit: Tissue ---3 ppb; Duck---5ppb; Raw Milk---1ppb.
Cross Reactivity: Benzylpenicillin ---100%; Ampicillin ---55%; Amoxicillin----620%; Nafcillin---15%; Oxacillin---44%; Cloxacillin---39%; Dicloxacillin ---73% Azlocillin---91%; Methicillin---32%; Cephalexin---1%.
Sample Recovery Rate: 90%±30%
Kits Components:
Item Specifications
ELISA Microtiter plate 96 wells
Standard Liquid 1 mL each (0 ppb, 0.3 ppb, 0.9 ppb, 2.7 ppb, 8.1 ppb, 24.3 ppb)
HRP Conjugate 12 mL
Antibody Working Solution 7 mL
Substrate Reagent A 7 mL
Substrate Reagent B 7 mL
Stop Solution 7 mL
20×Concentrated Wash Buffer 25 mL
5×Concentrated Sample Solution 1 40 mL
50×Concentrated Sample Solution 2 10 mL
Plate Sealer 3 pieces
Sealed Bag 1 piece
Manual 1 copy
Instruments: Microplate reader, Printer, Homogenizer, Oscillators, Centrifuge, Graduated pipette, Balance (sensibility 0.01g).
Micropipette: Single channel (20-200 uL, 100-1000 uL), Multichannel (30-300 uL).
Reagents: ZnSO4·7H2O
Storage And Expiry Date: Store the kit at 2~8°C. Do not freeze any test kit components. Return any unused microwells to their original foil bag and reseal them together with the desiccant provided and further store at 2 - 8°C. Expiry date: expiration date is on the packing box.