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Bovine Transforming Growth Factor Beta 2 (TGFb2) ELISA Kit

SKU:
BODL00165
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P21214
Sensitivity:
5.8pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
TGF-B2,TGF-Beta2,G-TSF,LAP,Cetermin,Polyergin,Latency-associated peptide,BSC-1 cell growth inhibitor,Glioblastoma-derived T-cell suppressor factor
Reactivity:
Bovine
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
Frequently bought together:

Description

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Bovine Transforming Growth Factor Beta 2 (TGFb2) ELISA Kit

The Bovine Transforming Growth Factor Beta 2 (TGFB2) ELISA Kit is specifically designed for the precise measurement of TGFB2 levels in bovine serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research purposes.Transforming Growth Factor Beta 2 (TGFB2) is a key protein involved in cell growth, differentiation, and immune response regulation. It plays a critical role in various biological processes, including tissue repair, embryonic development, and immune function.

Understanding the levels of TGFB2 can provide valuable insights into different diseases such as cancer, fibrosis, and autoimmune disorders, making it a valuable tool for research and therapeutic development.Overall, the Bovine TGFB2 ELISA Kit is a reliable and efficient solution for studying the role of TGFB2 in various biological processes and disease conditions in bovine models.

Product Name:Bovine Transforming Growth Factor Beta 2 (TGFb2) ELISA Kit
Product Code:BODL00165
Size:96 Assays
Target:TGFb2
Synonyms:TGF-B2, TGF-Beta2, G-TSF, LAP, Cetermin, Polyergin, Latency-associated peptide, BSC-1 cell growth inhibitor, Glioblastoma-derived T-cell suppressor factor
Detection Method:Sandwich
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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