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Bovine Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) ELISA Kit

SKU:
BODL00172
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P79121
Sensitivity:
0.118ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
SFD,TIMP Metallopeptidase Inhibitor 3,Metallopeptidase Inhibitor 3,Sorsby Fundus Dystrophy,Pseudoinflammatory
Reactivity:
Bovine
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€749
Frequently bought together:

Description

system_update_altDatasheet

Bovine Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) ELISA Kit

The Bovine Tissue Inhibitors of Metalloproteinase-3 (TIMP3) ELISA Kit is a valuable tool for the precise measurement of TIMP3 levels in bovine tissues, serum, plasma, or cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.TIMP3 is a key inhibitor of metalloproteinases, enzymes that play a critical role in extracellular matrix degradation and tissue remodeling.

Dysregulation of TIMP3 has been implicated in various diseases, including cancer, cardiovascular disorders, and inflammatory conditions. The ability to quantify TIMP3 levels can provide important insights into disease mechanisms and aid in the development of potential therapeutic interventions.With its reliable performance and broad versatility, the Bovine TIMP3 ELISA Kit is an indispensable tool for researchers studying the role of TIMP3 in health and disease.

Product Name:Bovine Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) ELISA Kit
Product Code:BODL00172
Size:96 Assays
Target:TIMP3
Synonyms:SFD, TIMP Metallopeptidase Inhibitor 3, Metallopeptidase Inhibitor 3, Sorsby Fundus Dystrophy, Pseudoinflammatory
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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