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Bovine Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) ELISA Kit

SKU:
BODL00170
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P20414
Sensitivity:
0.92ng/mL
Range:
2.343-150ng/mL
ELISA Type:
Sandwich
Synonyms:
EPO,CLGI,EPA,HCI,Erythroid Potentiating Activity,Collagenase Inhibitor,Metalloproteinase inhibitor 1,Fibroblast collagenase inhibitor
Reactivity:
Bovine
Sample Type:
Serum, Plasma And Other Biological Fluids
$899
Frequently bought together:

Description

system_update_altDatasheet

Bovine Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) ELISA Kit

The Bovine Tissue Inhibitors of Metalloproteinase-1 (TIMP1) ELISA Kit is a powerful tool for the precise detection of TIMP1 levels in bovine serum, plasma, and tissue lysate samples. With exceptional sensitivity and specificity, this kit delivers accurate and reproducible results, making it ideal for a variety of research applications.TIMP1 is a key regulator of metalloproteinases, enzymes involved in tissue remodeling, inflammation, and wound healing. Abnormal TIMP1 levels have been associated with various diseases such as cancer, fibrosis, and inflammatory disorders, highlighting its importance as a biomarker for disease progression and potential therapeutic targets.

By utilizing the Bovine TIMP1 ELISA Kit, researchers can gain valuable insights into the role of TIMP1 in various physiological and pathological processes, ultimately contributing to the development of novel diagnostic tools and treatment strategies.

Product Name:Bovine Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) ELISA Kit
Product Code:BODL00170
Size:96 Assays
Target:TIMP1
Synonyms:EPO, CLGI, EPA, HCI, Erythroid Potentiating Activity, Collagenase Inhibitor, Metalloproteinase inhibitor 1, Fibroblast collagenase inhibitor
Detection Method:Sandwich
Range:2.343-150ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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