Bovine T Cell Response Array (10 targets) (SARB0006)
- SKU:
- SARB0006
- Product Type:
- Protein Array
- Analytes:
- CD40 Ligand (TNFSF5)
- IFN-gamma
- IL-1 alpha (IL-1 F1)
- IL-1 beta (IL-1 F2)
- IL-10
- IL-13
- IL-17A
- IL-21
- NCAM-1 (CD56)
- TNF alpha
- Reactivity:
- Bovine
- Applications:
- Multiplex Array
Description
Bovine T Cell Response Array (10 targets) (SARB0006)
The Bovine T-Cell Response Array (SARB0006) is a comprehensive tool for studying the immune response in bovine species. This array includes probes for 10 targets involved in T-cell activation, proliferation, and differentiation, providing researchers with valuable insights into the bovine immune system.With its high sensitivity and specificity, this array allows for the simultaneous detection and quantification of multiple T-cell response markers in bovine samples.
This makes it an ideal solution for studies on immune responses to pathogens, vaccines, or other stimuli in bovine models.By utilizing the Bovine T-Cell Response Array, researchers can uncover valuable information about the regulation of T-cell responses in bovine species, leading to a better understanding of immune mechanisms and potential therapeutic targets for diseases affecting these animals.
| Product Sku: | SARB0006 |
| Size: | 2, 4, or 8 |
| Species Detected: | Bovine |
| Number of Targets Detected: | 10 |
| Gene Symbols: | CD40LG, IFNG, IL10, IL13, IL17A, IL1A, IL1B, IL21, NCAM1, TNF |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | MAPK Signaling / NFkB Signaling |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
CD40 Ligand (TNFSF5)
| IFN-gamma
| IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| IL-10
|
IL-13
| IL-17A
| IL-21
| NCAM-1 (CD56)
| TNF alpha
|
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Bovine T Cell Response Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
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