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Bovine Stem Cell Factor (SCF) ELISA Kit

SKU:
BODL00157
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q28132
Sensitivity:
19.6pg/mL
Range:
78.125-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
KITLG,MGF,SF,KL1,Kitl,FPH2,Kit-Ligand,Steel Factor,Mast Cell Growth Factor,Steel factor,Familial Progressive Hyperpigmentation 2
Reactivity:
Bovine
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates And Other Biological Fluids
€749
Frequently bought together:

Description

system_update_altDatasheet

Bovine Stem Cell Factor (SCF) ELISA Kit

The Bovine Stem Cell Factor (SCF) ELISA Kit is a valuable tool for measuring levels of SCF in bovine serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, delivering precise and consistent results for various research purposes.SCF is a key factor in the regulation of stem cell proliferation, differentiation, and survival. By accurately quantifying SCF levels, researchers can gain insights into the mechanisms underlying stem cell function and potential applications in regenerative medicine and cell therapy.

This ELISA kit is an essential resource for studying stem cells and their therapeutic potential in veterinary research and agriculture.Don't miss out on the opportunity to explore the role of SCF in bovine stem cell biology with the reliable and easy-to-use Bovine Stem Cell Factor (SCF) ELISA Kit. Order now and advance your research efforts in stem cell science and veterinary medicine.

Product Name:Bovine Stem Cell Factor (SCF) ELISA Kit
Product Code:BODL00157
Size:96 Assays
Target:SCF
Synonyms:KITLG, MGF, SF, KL1, Kitl, FPH2, Kit-Ligand, Steel Factor, Mast Cell Growth Factor, Steel factor, Familial Progressive Hyperpigmentation 2
Detection Method:Sandwich
Range:78.125-5000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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