Bovine S100A1 Calcium Binding Protein (S100A1) ELISA Kit
The Bovine S100A1 (Calcium Binding Protein S100A1) ELISA Kit is specifically designed for the accurate quantification of S100A1 levels in bovine serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures precise and reliable results for a variety of research applications.S100A1 is a calcium-binding protein that plays a crucial role in various cellular processes, including the regulation of calcium homeostasis and cell differentiation.
Dysregulation of S100A1 has been implicated in a range of diseases, such as cardiovascular disorders and neurodegenerative conditions, making it a valuable biomarker for studying these pathologies and exploring potential therapeutic interventions.With this advanced ELISA kit, researchers can confidently analyze S100A1 levels in bovine samples, providing valuable insights into the underlying mechanisms of disease and facilitating the development of targeted treatments.
Product Name:
Bovine S100A1 Calcium Binding Protein (S100A1) ELISA Kit
Product Code:
BODL00153
Size:
96 Assays
Target:
S100A1
Synonyms:
S100-A1, S100A1, S100-Alpha, S-100 Protein, S-100 protein subunit alpha, S-100 protein alpha chain
Detection Method:
Sandwich
Range:
78.1-5,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
91-102
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
