Bovine S100 Calcium Binding Protein A9 (S100A9) ELISA Kit
The Bovine S100 Calcium Binding Protein A9 (S100A9) ELISA Kit from Assay Genie is a highly reliable and sensitive kit for the detection of S100A9 levels in bovine serum, plasma, and cell culture supernatants. This kit provides accurate and reproducible results, making it an essential tool for researchers studying inflammatory processes, immune responses, and other biological pathways where S100A9 plays a crucial role.S100A9 is a calcium-binding protein that plays a key role in inflammation, immune system regulation, and wound healing. Elevated levels of S100A9 have been associated with various pathological conditions, including autoimmune diseases, cancer, and inflammatory disorders.
By accurately measuring S100A9 levels, researchers can gain valuable insights into the underlying mechanisms of these diseases and potentially identify new therapeutic targets.Overall, the Bovine S100A9 ELISA Kit offers a convenient and reliable solution for studying S100A9 biology and its implications in various disease processes. Get accurate and specific results with this high-quality ELISA kit from Assay Genie.
Product Name:
Bovine S100 Calcium Binding Protein A9 (S100A9) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
91-102
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
