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Bovine S100 Calcium Binding Protein A8 (S100A8) ELISA Kit

SKU:
BODL00154
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P28782
Sensitivity:
32pg/mL
Range:
78.125-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
MRP8,MIF,60B8AG,CAGA,CFAG,CGLA,CP-10,L1Ag,NIF,P8,Calgranulin A,Cystic fibrosis antigen,Leukocyte L1 complex light chain,Migration inhibitory factor-related protein 8
Reactivity:
Bovine
Sample Type:
Serum, Plasma And Other Biological Fluids
$899
Frequently bought together:

Description

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Bovine S100 Calcium Binding Protein A8 (S100A8) ELISA Kit

The Bovine S100 Calcium Binding Protein A8 (S100A8) ELISA Kit is specifically designed for the precise quantification of S100A8 levels in bovine serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it an excellent choice for a variety of research applications.S100A8 is a critical calcium-binding protein that plays a key role in the immune response and inflammation.

Its expression is often elevated in various inflammatory conditions and diseases, making it a valuable biomarker for studying these processes and developing targeted therapies.Overall, the Bovine S100 Calcium Binding Protein A8 (S100A8) ELISA Kit is an essential tool for researchers looking to investigate the role of S100A8 in bovine health and disease.

Product Name:Bovine S100 Calcium Binding Protein A8 (S100A8) ELISA Kit
Product Code:BODL00154
Size:96 Assays
Target:S100A8
Synonyms:MRP8, MIF, 60B8AG, CAGA, CFAG, CGLA, CP-10, L1Ag, NIF, P8, Calgranulin A, Cystic fibrosis antigen, Leukocyte L1 complex light chain, Migration inhibitory factor-related protein 8
Detection Method:Sandwich
Range:78.1-5,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)91-10296
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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