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Bovine RAC-alpha serine/threonine-protein kinase (AKT1) ELISA Kit (BOEB0190)

SKU:
BOEB0190
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q01314
ELISA Type:
Sandwich
Reactivity:
Bovine
€699
Frequently bought together:

Description

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Bovine RAC-alpha serine/threonine-protein kinase (AKT1) ELISA Kit

The Bovine Rac Alpha Serine/Threonine Protein Kinase Akt1 ELISA Kit is specifically designed for the precise measurement of Rac Alpha Serine/Threonine Protein Kinase Akt1 levels in bovine serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it an invaluable tool for various research applications.Rac Alpha Serine/Threonine Protein Kinase Akt1 is a key protein kinase involved in numerous cellular processes, including cell survival, growth, and metabolism. Dysregulation of Akt1 has been implicated in various diseases, such as cancer, diabetes, and cardiovascular disorders, highlighting its significance as a potential biomarker for disease research and therapeutic development.

With the Bovine Rac Alpha Serine/Threonine Protein Kinase Akt1 ELISA Kit, researchers can confidently study the role of Akt1 in bovine physiology and pathology, leading to a better understanding of disease mechanisms and the exploration of novel treatment options. Trust in this advanced ELISA kit for accurate and reliable Akt1 quantification in bovine samples.

Product Name:Bovine RAC-alpha serine/threonine-protein kinase (AKT1) ELISA Kit
SKU:BOEB0190
Size:96T
Target:Bovine RAC-alpha serine/threonine-protein kinase (AKT1)
Synonyms:Protein kinase B, Protein kinase B alpha, RAC-PK-alpha, PKB, PKB alpha, PKB
Assay Type:Sandwich
Detection Method:ELISA
Reactivity:Bovine
Detection Range:0.312-20ng/mL
Sensitivity:0.099ng/mL
Intra CV:Provided with the Kit
Inter CV:Provided with the Kit
Linearity:Provided with the Kit
Recovery:Provided with the Kit
Function:AKT1-specific substrates have been recently identified, including palladin (PALLD), which phosphorylation modulates cytoskeletal organization and cell motility; prohibitin (PHB), playing an important role in cell metabolism and proliferation; and CDKN1A, for which phosphorylation at 'Thr-145' induces its release from CDK2 and cytoplasmic relocalization. These recent findings indicate that the AKT1 isoform has a more specific role in cell motility and proliferation. Phosphorylates CLK2 thereby controlling cell survival to ionizing radiation. Phosphorylates SRPK2 and enhances its kinase activity towards SRSF2 and ACIN1 and promotes its nuclear translocation.
Uniprot:Q01314
Sample Type:Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Specificity:Natural and recombinant bovine RAC-alpha serine/threonine-protein kinase
Sub Unit:Interacts (via the C-terminus) with CCDC88A (via its C-terminus) and THEM4 (via its C-terminus). Interacts with AKTIP. Interacts (via PH domain) with MTCP1, TCL1A AND TCL1B. Interacts with TRAF6. Interacts with GRB10; the interaction leads to GRB10 phosphorylation thus promoting YWHAE binding. Interacts with RARA; the interaction phosphorylates RARA and represses its transactivation activity. Interacts with MAP3K5 and TNK2. Interacts with BAD, CLK2, PPP2R5B, STK3 and STK4. Interacts (via PH domain) with SIRT1. Interacts with SRPK2 in a phosphorylation-dependent manner. Interacts with RAF1. Interacts with PKN2 (via C-terminal domain); the interaction occurs with the C-terminus cleavage products of PKN2 in apoptotic cells. Interacts with TRIM13; the interaction ubiquitinates AKT1 leading to its proteasomal degradation. Interacts with and phosphorylated by PDPK1. Interacts with BTBD10. Interacts with KCTD20. Interacts with PA2G4. Interacts with PA2G4. Interacts with KIF14; the interaction is detected in the plasma membrane upon INS stimulation and promotes AKT1 phosphorylation. Interacts with FAM83B; activates the PI3K/AKT signaling cascade. Interacts with WDFY2 (via WD repeats 1-3). Forms a complex with WDFY2 and FOXO1. Interacts with FAM168A.
Research Area:Cancer
Subcellular Location:Cytoplasm Nucleus Cell membrane Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A (By similarity). Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus. Colocalizes with WDFY2 in intracellular vesicles (By similarity).
Storage:Please see kit components below for exact storage details
Note:For research use only
UniProt Protein Function:Akt1: an oncogenic AGC kinase that plays a critical role in regulating cell survival and metabolism in many different signaling pathways. Dual phosphorylation is required for its activation. T308 is phosphorylated by PDK1 in the PI3 kinase pathway, and S473 is phosphorylated by mTOR in the mTORC2 pathway. The 'Lys-63'-linked ubiquitination of AKT1 by TRAF6 is important for its translocation to the plasma membrane, phosphorylation, and activation. When Akt is fully phosphorylated it translocates into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its proteosomal degradation. Hyperactive or overexpressed in a number of cancers including breast, prostate, lung, pancreatic, liver, ovarian and colorectal. Over 160 protein substrates are known including many that regulate transcription, metabolism, apoptosis, cell cycle, and growth.Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; EC 2.7.11.1; Oncoprotein; AGC group; AKT familyCellular Component: cytoplasm; intracellular; nucleus; plasma membraneMolecular Function: ATP binding; protein binding; protein serine/threonine kinase activityBiological Process: carbohydrate transport; glucose metabolic process; glycogen biosynthetic process; negative regulation of caspase activity; peptidyl-serine phosphorylation; phosphorylation; positive regulation of endothelial cell proliferation; protein amino acid phosphorylation; regulation of cell migration; regulation of translation
UniProt Protein Details:
NCBI Summary:
UniProt Code:Q01314
NCBI GenInfo Identifier:27806747
NCBI Gene ID:280991
NCBI Accession:NP_776411.1
UniProt Secondary Accession:Q01314,Q5ER96
UniProt Related Accession:Q01314
Molecular Weight:55,748 Da
NCBI Full Name:RAC-alpha serine/threonine-protein kinase
NCBI Synonym Full Names:
NCBI Official Symbol:AKT1
NCBI Official Synonym Symbols:AKT
NCBI Protein Information:RAC-alpha serine/threonine-protein kinase
UniProt Protein Name:RAC-alpha serine/threonine-protein kinase
UniProt Synonym Protein Names:Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; RAC-PK-alpha
Protein Family:RAC serine/threonine-protein kinase
UniProt Gene Name:AKT1
UniProt Entry Name:AKT1_BOVIN
Component Quantity (96 Assays) Storage
ELISA Microplate (Dismountable) 8×12 strips -20°C
Lyophilized Standard 2 -20°C
Sample Diluent 20ml -20°C
Assay Diluent A 10mL -20°C
Assay Diluent B 10mL -20°C
Detection Reagent A 120µL -20°C
Detection Reagent B 120µL -20°C
Wash Buffer 30mL 4°C
Substrate 10mL 4°C
Stop Solution 10mL 4°C
Plate Sealer 5 -

Other materials and equipment required:

  • Microplate reader with 450 nm wavelength filter
  • Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
  • Incubator
  • Deionized or distilled water
  • Absorbent paper
  • Buffer resevoir

*Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

Allow all reagents to reach room temperature (Please do not dissolve the reagents at 37°C directly). All the reagents should be mixed thoroughly by gently swirling before pipetting. Avoid foaming. Keep appropriate numbers of strips for 1 experiment and remove extra strips from microtiter plate. Removed strips should be resealed and stored at -20°C until the kits expiry date. Prepare all reagents, working standards and samples as directed in the previous sections. Please predict the concentration before assaying. If values for these are not within the range of the standard curve, users must determine the optimal sample dilutions for their experiments. We recommend running all samples in duplicate.

Step
1. Add Sample: Add 100µL of Standard, Blank, or Sample per well. The blank well is added with Sample diluent. Solutions are added to the bottom of micro ELISA plate well, avoid inside wall touching and foaming as possible. Mix it gently. Cover the plate with sealer we provided. Incubate for 120 minutes at 37°C.
2. Remove the liquid from each well, don't wash. Add 100µL of Detection Reagent A working solution to each well. Cover with the Plate sealer. Gently tap the plate to ensure thorough mixing. Incubate for 1 hour at 37°C. Note: if Detection Reagent A appears cloudy warm to room temperature until solution is uniform.
3. Aspirate each well and wash, repeating the process three times. Wash by filling each well with Wash Buffer (approximately 400µL) (a squirt bottle, multi-channel pipette,manifold dispenser or automated washer are needed). Complete removal of liquid at each step is essential. After the last wash, completely remove remaining Wash Buffer by aspirating or decanting. Invert the plate and pat it against thick clean absorbent paper.
4. Add 100µL of Detection Reagent B working solution to each well. Cover with the Plate sealer. Incubate for 60 minutes at 37°C.
5. Repeat the wash process for five times as conducted in step 3.
6. Add 90µL of Substrate Solution to each well. Cover with a new Plate sealer and incubate for 10-20 minutes at 37°C. Protect the plate from light. The reaction time can be shortened or extended according to the actual color change, but this should not exceed more than 30 minutes. When apparent gradient appears in standard wells, user should terminatethe reaction.
7. Add 50µL of Stop Solution to each well. If color change does not appear uniform, gently tap the plate to ensure thorough mixing.
8. Determine the optical density (OD value) of each well at once, using a micro-plate reader set to 450 nm. User should open the micro-plate reader in advance, preheat the instrument, and set the testing parameters.
9. After experiment, store all reagents according to the specified storage temperature respectively until their expiry.

When carrying out an ELISA assay it is important to prepare your samples in order to achieve the best possible results. Below we have a list of procedures for the preparation of samples for different sample types.

Sample Type Protocol
Serum If using serum separator tubes, allow samples to clot for 30 minutes at room temperature. Centrifuge for 10 minutes at 1,000x g. Collect the serum fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. If serum separator tubes are not being used, allow samples to clot overnight at 2-8°C. Centrifuge for 10 minutes at 1,000x g. Remove serum and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.
Plasma Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples at 4°C for 15 mins at 1000 × g within 30 mins of collection. Collect the plasma fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. Note: Over haemolysed samples are not suitable for use with this kit.
Urine & Cerebrospinal Fluid Collect the urine (mid-stream) in a sterile container, centrifuge for 20 mins at 2000-3000 rpm. Remove supernatant and assay immediately. If any precipitation is detected, repeat the centrifugation step. A similar protocol can be used for cerebrospinal fluid.
Cell culture supernatant Collect the cell culture media by pipette, followed by centrifugation at 4°C for 20 mins at 1500 rpm. Collect the clear supernatant and assay immediately.
Cell lysates Solubilize cells in lysis buffer and allow to sit on ice for 30 minutes. Centrifuge tubes at 14,000 x g for 5 minutes to remove insoluble material. Aliquot the supernatant into a new tube and discard the remaining whole cell extract. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Tissue homogenates The preparation of tissue homogenates will vary depending upon tissue type. Rinse tissue with 1X PBS to remove excess blood & homogenize in 20ml of 1X PBS (including protease inhibitors) and store overnight at ≤ -20°C. Two freeze-thaw cycles are required to break the cell membranes. To further disrupt the cell membranes you can sonicate the samples. Centrifuge homogenates for 5 mins at 5000xg. Remove the supernatant and assay immediately or aliquot and store at -20°C or -80°C.
Tissue lysates Rinse tissue with PBS, cut into 1-2 mm pieces, and homogenize with a tissue homogenizer in PBS. Add an equal volume of RIPA buffer containing protease inhibitors and lyse tissues at room temperature for 30 minutes with gentle agitation. Centrifuge to remove debris. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Breast Milk Collect milk samples and centrifuge at 10,000 x g for 60 min at 4°C. Aliquot the supernatant and assay. For long term use, store samples at -80°C. Minimize freeze/thaw cycles.

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